AOAC 133rd Annual Meeting - Final Program

Poster Abstracts | Wednesday

P-W-039 Charles Yang , Thermo Fisher Scientific, San Jose, CA, USA; Illaria Palini , Silvia Bani , ISVEA, Poggibonsi, Italy; Debora D’Addona , Thermo Fisher Scientific, Milan, Italy Fast Analysis of Multi-Class Pesticides Panel in Wine and Olive Oil Extracts Using a Single Run LC-Triple Quadrupole Mass Spectrometry Increasing food safety concerns and the growing agricultural trade has resulted in stringent pesticide regulations globally. To comply with regulatory standards, screening methods for large numbers of pesticides is becoming important. Tandem quad- rupole mass spectrometry offers highly sensitive, specific and selective detection in complex matrices. This poster describes a method for analysis of multi-class pesticides for wine and olive oil using liquid chromatography coupled with triple quad- rupole mass spectrometer. An Accucore aQ 2.1 × 100 mm, 2.6 µ column was used for the separation of analytes within 15mins. A mobile phase of 0.1% formic acid/5 mM ammo- nium formate in water and in methanon with injection size of 1 uL. Detection was performed with TSQ Quantis triple quad- rupole mass spectrometer coupled to Vanquish Flex uHPLC. Samples were extracted with organic solvent using QuEChERS method and lipid removal cartridge. A multi-residue method was developed for approximately 300 pesticides with polar- ity switching. Ion ratio confirmation of ±30%, RT accuracy of ±0.1min to show robustness of the method required by EU SANTE Guidance11813_2017. The method was applied to both wine and olive oil extract. The results show excellent LOQ / LOD between 0.5 to10ppb, while reproducibility and precision n=8 showed excellent results and linearity with R2=0.9900. The lipid removal cartridge showed good %Rec between 10 and 50 ppb within 70-120% of the SANTE Guidance. Presenter: Charles Yang, Thermo Fisher Scientific, San Jose, CA, USA, Email: charles.yang@thermofisher.com P-W-040 Hiroyuki Kikuchi , National Institute of Health Sciences, Kanagawa, Japan; Takatoshi Sakai , Tomoko Okura , Satoru Nemoto , Hiroshi Akiyama , National Institute of Health Sciences, Kawasaki City, Japan A Simple and Sensitive LC-MS/MS Method for Determining Residues of the Tranquilizer Chlorpromazine in Livestock Products, Seafood, and Honey A simple and sensitive analytical method for determining resi- dues of the tranquilizer chlorpromazine in foods such as livestock products, seafood, and honey was developed. The method involves solvent extraction with acetone, clean-up using InertSep MC-1 strong cation exchange solid-phase extraction cartridges, and LC-MS/MS analysis with selective reaction monitoring in negative ionization mode. Chlorpromazine decreased over time in the extraction step when using methanol or ethanol as the extraction solvent. Therefore, we examined the stability of chlorpromazine in the presence of various solvents. Acetone was selected as the extraction solvent because chlorpromazine did not decrease over time in acetone extracts. Furthermore, because chlorpromazine adsorbs into glass surfaces, polypropylene

India; Robert Clifford , Shimadzu Scientific Instruments, Inc., Columbia, MD, USA Multiresidue Analysis of Pesticides in Turmeric ( Curcuma Longa ) Powder by GCMS/MS Using QuEChERS Extraction Method Turmeric is a plant indigenous to south Asia, with majority of its production coming from India. The rhizome of the plant is used to produce powdered turmeric which is used in food, cosmetics, and some medicines. It is one of the most essential spices used extensively in Indian cooking. The intensive use of agrochemicals during its cultivation has given rise to concerns over consumer’s exposure to pesticide residues and associated health risks. Hence, it is important for turmeric producers and regulators to ensure that presence of residual pesticides meet compliance criteria. The Minimum Residual Limit (MRL) as per EU guideline ranges from 5 to 5000 ppb level. This study reports a sensitive and reliable analytical method for quantitative analysis of multi-pesticide residues in commercially available turmeric powder by using GCMS/MS triple quadrupole system. Presenter: Sunil Singh, Shimadzu Analytical (India) Pvt. Ltd, Mumbai, India, Email: nmlock@shimadzu.com P-W-038 Thomas Hektor , R-Biopharm AG, Darmstadt, Germany; Nermin Sajic , Michalina Oplatowska-Stachowiak , R-Biopharm Nederland B.V., Arnhem, Netherlands Rapid and Sensitive Malachite Green Total ELISA Triphenylemethane dyes such as malachite green (MG) and crystal violet (CV) are organic dyes widely used in industry for dying purposes. They have been also used in aquacultures to treat and prevent fungal and parasitic infections in fish and shrimp. However, due to their genotoxic and carcinogenic effects, they have never been authorised to be used in aquacul- ture products for human consumption. These dyes are extensively absorbed and metabolised to the reduced leuco forms: leucoma- lachite green (LMG) and leucocrystal violet (LCV) that can persist in tissues for a long time. The detection of MG, LCV and their metabolites in fish or shrimp indicates illegal use of these dyes in aquacultures. In the European Union the minimum required performance limit for a method for the detection of a sum of MG and LMG is 2 μg/kg (Commission Decision 2002/657/EC). Malachite Green Total (MGT) ELISA was developed to provide a new method for simultaneous detection of residues of both MG and CV. The new test is based on a polyclonal antibody against MG and cross-reactivity with CV. The test is applicable for anal- ysis of fish and shrimp samples. The performance of the test was evaluated in Food Analysis Performance Assessment Scheme (FAPAS) scheme and the z-score was -0.8 for a fish sample containing both MG and LMG. MGT ELISA can be used as a fast, simple, and cost-effective screening tool for the detection of illegal use of both MG and CV in aquacultures. Presenter: Thomas Hektor, R-Biopharm AG, Darmstadt, Germany, Email: t.hektor@r-biopharm.de

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