AOAC 133rd Annual Meeting - Final Program

Poster Abstracts | Wednesday

P-W-043 Zhenhe Chen , Satoshi Yamaki , Jing Dong ,

tubes were used for the extraction step to prevent loss of recov- ery. The developed method was validated using eight food products spiked with chlorpromazine at 0.1 μg/kg. The valida- tion results exhibited excellent recovery (range, 86-106%) and precision (variation <10%). The limit of quantification (S/N≥10) of the developed method was 0.1 μg/kg. The proposed method would be very useful for regulatory monitoring of the illegal use of chlorpromazine in foods. Presenter: Hiroyuki Kikuchi, National Institute of Health Sciences, Kanagawa, Japan, Email: h-kikuchi@nihs.go.jp P-W-041 Zhao Liu , Qiang Li , Hongyuan Hao , Taohong Huang , Shimadzu China, Shanghai, China; Eberhardt Kuhn , Shimadzu USA, Columbia, MD, USA Simultaneous Determination of 130 Veterinary Drugs in Pork Using Ultra Performance Liquid Chromatograph- Tandem Mass Spectrometry It has been reported that veterinary drugs have been widely used in animal derived food production. However, it is diffi- cult to remove residues of veterinary drugs remained in food, especially antibiotics, which are the most commonly residues in meat product. In the long run, the residual status of antibiotics will be harmful to human beings, even to leading acute diseases. Therefore, it is imperative to establish efficacy methods to detect the concentration of residues in animal derived food to evaluate possible biological risks. In the present study, a rapid, sensitive and specific method was developed to quantitatively detect 130 veterinary drugs residues in pork, including sulfonamides, quinolones, tetracyclines and β -receptor agonists, etc. with ultra-performance liquid chromatograph-tandem mass spectrom- etry (LC-MS/MS). Presenter: Eberhardt Kuhn, Shimadzu USA, Columbia, MD, USA, Email: erkuhn@shimadzu.com P-W-042 Qiaoxia Liu , Qiang Li , Hongyuan Hao , Taohong Huang , Shimadzu China, Shanghai, China; Eberhardt Kuhn , Shimadzu USA, Columbia, MD, USA Qualitative and Quantitative Analysis of Pork in Beef Food with LC-MS/MS Food fraud is a problem on global scale, especially the meat fraud. Such as, horse meat was added in the beef food, and pork was used as beef. Consumers and the food industry are increasingly aware of this problem, and the specific detection of amounts of certain meat species in processed food is still prob- lematic. Presently, ELISA and PCR methods are routinely used for the species authentication. As we know, the ELISA method often results in false positive or false negative results, and the PCR method is not so accuracy for DNA being prone to degrada- tion. So the characteristic peptides multiple reaction monitoring (MRM) method by LC-MS/MS is a good choice for the qualita- tive and quantitative analysis of meat authentication. Presenter: Eberhardt Kuhn, Shimadzu USA, Columbia, MD, USA, Email: erkuhn@shimadzu.com

Yuki Hashi , Naoki Hamada , Shimadzu China, Shanghai, China; Eberhardt Kuhn , Shimadzu USA, Columbia, MD, USA Fast Screening of Pesticides in Foods and Agricultural Products with Probe Electrospray Ionization (PESI) Tandem Mass Spectrometry Pesticides screening in food and agricultural products have been intensively emphasized due to the increasing food safety requirement. Recently, high-resolution mass has been thought to a promising screening method, but it is very costly. Pesticides usually have broad polar range and both GCMS and LCMS methods should be used for their determination. Probe elec- trospray ionization (PESI) source, as an ambient ionization technique, which could be used for the determination of both polar and semi-polar compounds. Meanwhile, as an in situ ionization, PESI source usually needs less sample pretreatments. In this study, 80 pesticides are selected as research targets, which are commonly monitored in vegetables every year in China. A PESI-MS/MS approach is developed aiming at both high-throughput and quantification using MRM acquisition. Presenter: Eberhardt Kuhn, Shimadzu USA, Columbia, MD, USA, Email: erkuhn@shimadzu.com P-W-044 Biao Ren , Changkun Li , Yueqi Li , Shimadzu China, Beijing, China; Taohong Huang , Shimadzu China, Shanghai, China; Eberhardt Kuhn , Shimadzu USA, Columbia, MD, USA Rapid Screening and Quantitative Analysis of Pesticides in Vegetables by UHPLC-Q-TOF-MS/MS The method is simple and fast. After extraction by acetonitrile, samples are salted out, centrifuged, filtered, and then directly injected for analysis; the analysis speed is fast, the detection time is only 15 minutes; the sensitivity is high, the detection limit is 0.01-0.75 ng/mL; the linear relationship is good, the correlation coefficient r is greater than 0.99; the precision is good, RSD is less than 5%; the quality is excellent, accuracy and first-class quality. The accuracy of spectral mass number is less than 1 ppm, the isotope distribution is true and accurate, and the matching degree of secondary spectral library is high. The method is simple, rapid, sensitive, accurate and stable for qualitative and quantitative screening, and can be used for rapid qualitative screening and quantitative detection of pesti- cide residues in vegetables.In this paper, a rapid qualitative and quantitative method for the determination of 49 routine pesticides in vegetables by LCMS-9030 ultra-high performance liquid chromatography quadrupole time-of-flight mass spectrom- etry was established. Presenter: Eberhardt Kuhn, Shimadzu USA, Columbia, MD, USA, Email: erkuhn@shimadzu.com P-W-045 Jian Wang , Willis Chow , Daniel Leung , Canadian Food Inspection Agency (CFIA), Calgary, AB, Canada; Jon Wong , U.S. Food and Drug Administration, College Park, MD, USA; James Chang , Thermo Fisher Scientific, San Jose,

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