AOAC ERP MICRO AUGUST 2018

OMAMAN-44 B: Collaborative Study Protocol Expert Review Panel Use Only August 2018

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Bacillus Bacillus

cereus subtilis

ATCC 6464 ATCC 6633 ATCC 10580 ATCC 11509 ATCC 19432 ATCC 29212 ATCC 51559 ATCC 8043 ATCC 19418 ATCC 43195 ATCC 10538 ATCC 8293 ATCC 33090 ATCC BAA-139 ATCC 7644 ATCC 11289 ATCC 43549 ATCC 10240 ATCC 14909 QL 17041-1 QL 17041-3 ATCC 27844 ATCC 6538 ATCC 6302 ATCC 19615 ATCC 17802 QL 021111A

Soil

Not Available Lung of dog

Bordetella Brochothrix Enterococcus Enterococcus Enterococcus Enterococcus Haemophilus

bronchiseptica thermosphacta

Animal-derived foodstuff

durans faecalis faecium

Not Available

Urine

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Clinical isolate Not Available Not Available

hirae

influenzae

Kurthia Kurthia Listeria Listeria Listeria Listeria Listeria

gibsonii

meat

zopfii

Not Available fermenting olives

Leuconostoc

mesenteroides

innocua ivanovii

cow brain

washing water

monocytogenes

1/2 C

seeligeri

6B 6B air

welshimeri

Micrococcus Pseudomonas Pseudomonas Pseudomonas Staphylococcus Staphylococcus Streptococcus Streptococcus

luteus

alcaligenes

swimming-pool water

extremorientalis

Raw Milk Isolate Raw Milk Isolate Human skin human lesion Not Available

fluorescens

hominis aureus

pneumoniae

pyogenes

Pharynx of child following sore throat.

Vibrio Vibrio

parahaemolyticus

Shirasu food poisoning

vulnificus Seafood Product a ATCC-American Type Culture Collection, Manassas, VA; b QL-Q Laboratories Culture Collection, Cincinnati,

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OH.

5.0 5 6 Eight different food matrices, and two environmental samples, will be evaluated by Peel Plate EB and by 7 an appropriate reference method (Table 3). The stainless steel, chicken rinse with neutralizing buffered 8 peptone water (n-BPW), pasteurized milk, non-fat dry milk, probiotic infant cereal and probiotic infant 9 formula (milk based) will be analyzed by Q-laboratories. The butter, vanilla ice cream, infant formula 10 (soy based), non-probiotic cereal and chicken rinse with BPD will be analyzed by manufacturer. The 11 matrices will be validated according to Appendix J requirements (minimum of 3 contamination levels, 5 12 replicate portions tested from each level, plus an uncontaminated level, if appropriate). See Table 3 for 13 recommended contamination levels. Naturally contaminated product may be mixed with 14 uncontaminated product to achieve appropriate levels. If naturally contaminated product cannot be 15 obtained for any of the matrices, then artificially contaminated lots can be prepared in accordance with 16 the recommended contamination levels. For artificially contaminated matrices, the uncontaminated 17 level must be included. 18 19 NOTE : Pasteurized whole milk, butter, non-fat dry milk, probiotic infant formula, non-probiotic infant 20 formula and infant cereal w/o probiotics will be evaluated for AOAC and MicroVal review. Chicken 21 ERP Use Only Matrix Study – SLV