AOAC ERP MICRO AUGUST 2018

OMAMAN-44 B: Collaborative Study Protocol Expert Review Panel Use Only August 2018

carcass rinse with n-BPW and stainless steel environmental surface sponge samples will be evaluated for 1 AOAC purposes only by the outside laboratory. Manufacturer is supplementing data to complete AOAC 2 matrix claim criteria for butter, non-fat dry milk, infant formula (soy based), infant cereal, vanilla ice 3 cream and chicken rinse with BPD.

4 5

Table 3. Matrix Preparation

6

Inoculation Organisms a

Test Portion Peel Plate EB

Reference Method

Matrix

Target Contamination Level

Replicates

0 CFU/mL b

5

ISO 21528- 1:2017 (<100 CFU/mL) & ISO 21528-2:2017 (>100 CFU/mL ISO 21528- 1:2017 (<100 CFU/g) & ISO 21528-2:2017 (>100 CFU/g) ISO 21528- 1:2017 (<100 CFU/mL) & ISO 21528-2:2017 (>100 CFU/mL ISO 21528- 1:2017 (<100 CFU/mL) & ISO 21528-2:2017 (>100 CFU/mL ISO 21528- 1:2017 (<100 CFU/mL) & ISO 21528-2:2017 (>100 CFU/mL ISO 21528- 1:2017 (<100 CFU/mL) & ISO 21528-2:2017 (>100 CFU/mL

Enterobacter amnigenus ATCC 51816 (Heat Stressed)

10–100 CFU/mL

5

Pasteurized whole milk

25 mL

100–5,000 CFU/mL

5

5,000–100,000 CFU/mL

5

0 CFU/g

5

Serratia marcescens ATCC 13880 (Heat stressed)

10–100 CFU/g

5

Butter

25 g

100–5,000 CFU/g

5

5,000–100,000 CFU/g

5

0 CFU/mL

5

Hafnia alvei ATCC 51815

10–100 CFU/mL

5

Non-fat dry milk

25 mL

100–5,000 CFU/mL

5

5,000–100,000 CFU/mL

5

0 CFU/mL

5

Klebsiella oxytoca ATCC 700324

10–100 CFU/mL

5

Vanilla ice cream

25 mL

100–5,000 CFU/mL ERP Use Only 5 5,000–100,000 CFU/mL 5 0 CFU/mL 5 10–100 CFU/mL 5

Cronobacter sakazakii CCUG 28863

Probiotic liquid infant formula (milk based)

25 mL

100–5,000 CFU/mL

5

5,000–100,000 CFU/mL

5

0 CFU/mL

5

Non-probiotic liquid infant formula (soy based)

Enterobacter aerogenes ATCC 13048

10–100 CFU/mL

5

25 mL

100–5,000 CFU/mL

5

5,000–100,000 CFU/mL

5