AOAC ERP MICRO AUGUST 2018

OMAMAN-31 E: AOAC RI PTM Report 091501 ERP Use Only - March 2016

Table 4: Matrixes and Inoculating Organisms

MDA 2 Salmonella Testing Time Points

Inoculating Organism (Culture Condition) Salmonella Agona ATCC 1 51957

Matrix/Test Portion Size

# of Replicates

Reference Method (Enrichment)

Target Inoculum Level

0 CFU/ Test Portion 0.2-2 CFU/ Test Portion 2-5 CFU/ Test Portion 0 CFU/ Test Portion 0.2-2 CFU/ Test Portion 2-5 CFU/ Test Portion

5

Raw Ground Beef (73 % Lean) (25 g & 375 g) Raw Ground Chicken (25 g & 375 g) Chicken Carcass Rinse Chicken Carcass Sponge Instant Non-fat Dry Milk (25 g)

USDA/FSIS MLG 4.08 (mTSB) USDA/FSIS MLG 4.08 (BPW) USDA/FSIS MLG 4.08 (BPW) USDA/FSIS MLG 4.08 (BPW)

10 and 24 hours 14 and 24 hours

20

5 5

Salmonella Heidelberg NCTC 2 5717 Naturally Contaminated 3 Naturally Contaminated 3

20

5

Lot 1 & Lot 2 Lot 1 & Lot 2

20 & 20 20 & 20

18 hours

18 hours

0 CFU/ Test Portion 0.2-2 CFU/ Test Portion 2-5 CFU/ Test Portion 0 CFU/ Test Portion 0.2-2 CFU/ Test Portion 2-5 CFU/ Test Portion 0 CFU/ Test Portion 0.2-2 CFU/ Test Portion 2-5 CFU/ Test Portion 0 CFU/ Test Portion 0.2-2 CFU/ Test Portion 2-5 CFU/ Test Portion 0 CFU/ Test Portion 0.2-2 CFU/ Test Portion 2-5 CFU/ Test Portion 0 CFU/ Test Portion 0.2-2 CFU/ Test Portion 2-5 CFU/ Test Portion 0 CFU/ Test Portion 0.2-2 CFU/ Test Portion 2-5 CFU/ Test Portion 0 CFU/ Test Portion 0.2-2 CFU/ Test Portion 2-5 CFU/ Test Portion

5

FDA/BAM Chapter 5 (Brilliant Green Water) FDA/BAM Chapter 5 (TSB) FDA/BAM Chapter 5 (Reconstituted non- fat dry milk)

Salmonella Infantis ATCC 1 51741 (lyophilized)

20 hours

20

5 5

Salmonella Tallahassee ATCC 1 12002 (lyophilized)

Black Pepper (25 g)

20

18 hours

5 5 5 5

Salmonella Newport ATCC 1 6962 (lyophilized)

Cocoa Powder (25 g)

24 hours

20

Salmonella Enteriditis ATCC 1 13076 (heat-stressed)

Pasteurized Liquid Whole Egg (100 g) Raw Whole Shrimp (25 g)

USDA/FSIS MLG 4.08 (BPW)

20

18 hours

5 5

Salmonella Virchow ATCC 1 51955

FDA/BAM Chapter 5 (Lactose)

20

18 hours

5 5

Raw Bagged Spinach (25 g)

Salmonella Schwarzengrund STs 4 95

FDA/BAM Chapter 5 (Lactose)

20

18 hours

5 5 AOAC Research Institute Expert Review Panel Use Only Salmonella Saintpaul ATCC 1 9712 Salmonella Kentucky ATCC 1 9263 (heat-stressed) 18 hours FDA Guidance Document (BPW+n) 20 5 5 18 hours USDA/FSIS MLG 4.08 (BPW) 20 5

Spent Sprout Irrigation Water (375 mL) Cooked Breaded Chicken (325 g)

1 ATCC - American Type Culture Collection 2 NCTC-National Culture Type Collection 3 Natural contamination was present during the background screen: 2 separate lots evaluated (20 replicates each) 4 STs- Pennsylvania State University Culture Collection