AOAC ERP MICRO AUGUST 2018

OMAMAN-31 E: AOAC RI PTM Report 091501 ERP Use Only - March 2016

Table 4: Matrixes and Inoculating Organisms (continued)

MDA 2 Salmonella Testing Time Points

Inoculating Organism (Culture Condition) Salmonella Muenchen ATCC 1 BAA 1594 (heat-stressed)

Matrix/Test Portion Size

# of Replicates

Reference Method (Enrichment) FDA/BAM Chapter 5 (Lactose) FDA/BAM Chapter 5 (Lactose) FDA/BAM Chapter 5 (Lactose) FDA/BAM Chapter 5 (Lactose) FDA/BAM Chapter 5 (Lactose) FDA/BAM Chapter 5 (Lactose)

Target Inoculum Level

0 CFU/ Test Portion 0.2-2 CFU/ Test Portion 2-5 CFU/ Test Portion 0 CFU/ Test Portion 0.2-2 CFU/ Test Portion 2-5 CFU/ Test Portion 0 CFU/ Test Portion 0.2-2 CFU/ Test Portion

5

Creamy Peanut Butter (25 g & 325 g)

20

18 hours

5 5

Salmonella Typhimurium ATCC 1 14028 (lyophilized) Salmonella Montevideo ATCC 1 8387 (heat-stressed)

Dry Dog Food (25 g & 325 g)

20

18 hours

5 5

Pasteurized Processed American Cheese (25 g)

20

18 hours

2-5 CFU/ Test Portion

5

Salmonella Dublin STs 2 27 & Proteus vulgaris ATCC 1 6380 (x 10-100) Salmonella Kahla ATCC 1 17980

0 CFU/ 4” x 4” (100 cm 2 ) ~50 CFU/ 4” x 4” (100 cm 2 )

5

Sealed Concrete (225 mL)

20

18 hours

~100 CFU/ 4” x 4” (100 cm 2 ) 5 0 CFU/ 1” x 1” (5 cm 2 ) ~50 CFU/ 1” x 1” (5 cm 2 ) ~100 CFU/ 1” x 1” (5 cm 2 ) 0 CFU/ 4” x 4” (100 cm 2 ) ~50 CFU/ 4” x 4” (100 cm 2 ) 20 ~100 CFU/ 4” x 4” (100 cm 2 ) 5 5 20 5 5

Stainless Steel (10 mL) Sealed ceramic Tile (50 mL)

18 hours

Salmonella Anatum ATCC 1 9270

18 hours

1 ATCC- American Type Culture Collection 2 STs- Pennsylvania State University Culture Collection

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