AOAC ERP MICRO AUGUST 2018

B ird et al .: J ournal of AOAC I nternational V ol . 100, N o . 2, 2017  461

Table 2016.08D. Comparative results for the detection of L. monocytogenes at three inoculum levels in 25 g raw chicken breast fillet test portions by the 3M MDA 2 – L. monocytogenes method vs the USDA/FSIS MLG reference method in a collaborative study

Candidate presumptive

Candidate confirmed

Candidate result

Reference method

Candidate vs reference

n a

x b

Statistic Matrix Laboratory

POD CP n x

POD CC

POD C n x

POD R

dLPOD C

dLPOD CP

n x

Uninoculated control

Raw chicken breast fillet

1 2 3 4 5 6 7 8 9

12 0 0.00 12 0 12 0 0.00 12 0 12 0 0.00 12 0 12 0 0.00 12 0 12 0 0.00 12 0 12 0 0.00 12 0 12 0 0.00 12 0 12 0 0.00 12 0 12 0 0.00 12 0 12 0 0.00 12 2

0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.17 0.00 0.03 0.00 0.00 0.16 0.00 0.00 0.16 0.00 0.00 0.23 0.00 0.00

12 0 12 0 12 0 12 0 12 0 12 0 12 0 12 0 12 0 12 0

0.00 12 0 0.00 0.00 12 0 0.00 0.00 12 0 0.00 0.00 12 0 0.00 0.00 12 0 0.00 0.00 12 0 0.00 0.00 12 0 0.00 0.00 12 0 0.00 0.00 12 0 0.00 0.00 12 0 0.00 0.00 12 0 0.00 0.00 12 0 0.00 0.00 132 0 0.00

0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 NA

0.00 0.00 0.00 0.00 0.00 0.00 0.08 0.00 0.00

10 c

−0.17

11 NA d

NA NA NA NA

NA NA NA NA NA NA NA

NA

12 12 0 0.00 12 0 13 12 0 0.00 12 0

12 0 12 0

0.00 0.00 0.00

Estimate

All

132 0 0.00 132 0

0.00 132 0

 LCL e  UCL f

0.00 0.03 0.00 0.00 0.16 0.00 0.00 0.16 0.00 0.00 0.23

0.00 0.03 0.00 0.00 0.16 0.00 0.00 0.16 0.00 0.00 0.23

0.00 0.03 0.00 0.00 0.16 0.00 0.00 0.16 0.00 0.00 0.23

−0.03

−0.03

0.03

0.03

s r

 LCL  UCL

s L

 LCL  UCL

s R

 UCL  LCL

1.0000

1.0000

1.0000

1.0000

P T

Low inoculum level

1

12 7 0.58 12 7

0.58

12 7

0.58 12 7 0.58

0.00

0.00

Raw chicken breast fillet

12 9 ERP Use Only 0.75 12 7 0.58 12 10 0.83 12 5 0.42

2 3 4 5 6 7 8 9

12 9 0.75 12 9 12 10 0.83 12 10 12 9 0.75 12 9 12 8 0.66 12 8 12 9 0.75 12 9 12 7 0.58 12 6 12 7 0.58 12 7 12 8 0.66 12 9 12 6 0.50 12 8

0.75 0.83 0.75 0.66 0.75 0.50 0.58 0.75 0.66

0.17 0.41 0.42 0.22 0.15 0.00 0.00 0.08 0.08

0.00 0.00 0.00 0.00 0.08 0.16 0.00

12 9 12 8 12 9 12 6 12 7 12 8 12 6

0.75 12 4 0.33 0.66 12 5 0.42 0.75 12 6 0.50 0.50 12 6 0.50 0.58 12 7 0.58 0.66 12 7 0.58 0.50 12 5 0.42

−0.08 −0.16

10 c

11 NA NA NA NA NA 12 12 7 0.58 12 7 13 12 5 0.42 12 5

NA NA NA NA NA NA NA

NA

NA

0.58 0.42

12 7 12 5

0.58 12 4 0.42 0.42 12 6 0.50

0.16

0.00 0.00

−0.08

MPN, CFU/ test portion

Estimate 0.66

All

132 86 0.65 132 86

0.65 132 85 0.64 132 64 0.48

0.16 0.04 0.28

0.00

 LCL  UCL

0.51 0.83

0.57 0.73

0.57 0.73

0.56 0.73

0.40 0.57

−0.12

0.12