AOAC ERP MICRO AUGUST 2018

462 B ird et al .: J ournal of AOAC I nternational V ol . 100, N o . 2, 2017

Table 2016.07D. ( continued )

Candidate presumptive

Candidate confirmed

Candidate result

Reference method

Candidate vs reference

n a

x b

Statistic Matrix Laboratory

POD CP n x

POD CC

POD C n x

POD R

dLPOD C

dLPOD CP

n x

s r

0.48 0.43 0.52 0.00 0.00 0.17 0.48 0.43 0.52

0.48 0.43 0.52 0.00 0.00 0.18 0.48 0.43 0.52

0.48 0.43 0.52 0.00 0.00 0.18 0.49 0.43 0.52

0.51 0.46 0.52 0.00 0.00 0.14 0.51 0.46 0.52

 LCL  UCL

s L

 LCL  UCL

s R

 UCL  LCL

P T

0.7057

0.5632

0.6228

0.9192

High inoculum level

Raw chicken breast fillet

1

12 12 1.00 12 12

1.00

12 12 1.00 12 12 1.00

0.00

0.00

2 3 4 5 6 7 8 9

12 12 1.00 12 12 12 12 1.00 12 12 12 12 1.00 12 12 12 12 1.00 12 12 12 10 0.83 12 12 12 12 1.00 12 12 12 12 1.00 12 12 12 11 0.92 12 12 12 12 1.00 12 12

1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00

12 12 1.00 12 12 1.00 12 12 1.00 12 12 1.00 12 12 1.00 12 12 1.00 12 12 1.00 12 12 1.00 12 10 0.83 12 12 1.00 12 12 1.00 12 12 1.00 12 12 1.00 12 12 1.00 12 11 0.92 12 12 1.00 12 12 1.00 12 12 1.00

0.00 0.00 0.00 0.00 0.00 0.00 0.00

0.00 0.00 0.00 0.00 0.00 0.00 0.00

−0.08

−0.08

10 c

0.00

0.00

11 NA NA NA NA NA 12 12 12 1.00 12 12 13 12 12 1.00 12 12

NA NA NA NA NA NA NA

NA

NA

1.00 1.00

12 12 1.00 12 12 1.00 12 12 1.00 12 12 1.00

0.00 0.00

0.00 0.00

MPN, CFU/ test portion

Estimate 6.24

All

132 129 0.98 132 132

1.00 132 129 0.98 132 132 1.00

−0.02 −0.06

−0.02 −0.06

 LCL

3.58

0.93 0.99 0.15 0.13 0.17 0.03 0.00 0.08 0.15 0.13 0.18

0.97 1.00 0.00 0.00 0.16 0.00 0.00 0.16 0.00 0.00 0.23

0.93 0.99 0.15 0.13 0.17 0.03 0.00 0.08 0.15 0.13 0.18

0.97 1.00 0.00 0.00 0.16 0.00 0.00 0.16 0.00 0.00 0.23

 UCL 10.88

0.01

0.01

s r

 LCL  UCL

ERP Use Only

s L

 LCL  UCL

s R

 UCL  LCL

P T

0.1089

1.0000

0.1089

1.0000

a n = No. of samples tested. b x = No. of test samples with a positive result (presumptive or confirmed). c  Laboratory 10 results were not used in statistical analysis due to deviation from testing protocol. d  NA = Laboratory did not participate or submit data for this matrix.

e  LCL = Lower confidence limit. f  UCL = Upper confidence limit.

these concerned groups avoid handling this organism. After use, the enrichment medium and the 3M MDA 2 – Listeria monocytogenes tubes can potentially contain pathogenic materials. Periodically decontaminate laboratory benches and equipment (pipets, cap/decap tools, etc.) with a household

bleach solution (1–5%, v/v in water; 5250–6500 ppm) or DNA removal solution. When testing is complete, follow current industry standards for the disposal of contaminated waste. Consult the Material Safety Data Sheet for additional information and local regulations for disposal.