AOAC GFA Stakeholder Program Meeting Book (August 27, 2023)

CONCLUSIONS:

4. Claimed gluten sources for most of the commercial gluten ELISA kits used by the food industry or by independent third-party laboratories are wheat, barley and rye; however, the gluten content of barley and rye are overestimated in some of these kits, which have been able to be accredited under the ISO 17025 quality standard by many laboratories. These accredited methods (under the scope of “gluten in foods”) can produce inconsistent quantification of gluten residues in foods containing gluten from barley and rye. In this sense, influenced by the endorsement by Codex Alimentarius and by the Association of European Coeliac Societies (AOECS), ISO 17025–accredited laboratories with R5-sandwich-ELISA (Mendez method), Ridascreen Gliadin Kit (R7001), could be using a non-fit-for-purpose kit to quantify gluten in foods from barley and rye. These independent laboratories using a non-fit-for-purpose method, considered a reference method, could non-intentionally be discrediting more accurate results obtained in foods with barley and rye by other commercial LFD/ELISA gluten kits.

9 globalbrand@hygiena.com | August 21, 2023