AOAC SPIFAN Nutrients ERP-Final Review (Oct. 2018) Pubs

2011.06 (September 2017)-Fol-22 MLT Report

FOR ERP USE ONLY DO NOT DISTRIBUTE

The General MS parameters for API 5500 (Qtrap): Curtain gas(nitrogen):

25 psi;

Collision Assisted Dissociation

Medium;

IonSpray voltage:

5500 V;

Temperature:

700 °C;

Ion source gas1(zero air):

60 psi;

Ion source gas2(zero air):

50 psi;

Platform: Agilent 1290 Infinity Binary LC with Sciex 6500 (Q trap) LC- MS/MS system or 6500 Plus LC-MS/MS system: HPLC Conditions : Agilent 1290 Infinity Binary LC System: LC Column: Waters ACQUITY UPLC HSS T3 Column (100Å, 1.8 µm, 2.1 mm X 50 mm). Mobile Phase: A: 1% Glacial acetic acid and B: MS grade methanol as mobile phase components in different ratios at different time of the analysis. The Pumping gradient of two solvents at different time is as the following.

Flow (mL/min)

Time (min)

%B

0

0

0.5

0.5

0

0.5

5.00

40

0.5

5.05

100

0.5

6.50

100

0.5

6.55

0

0.5

9.00

0

0.5

A = 1% Glacial Acetic Acid in Water, B = 100% Methanol

Column Temperature: 40 ° C

Collaborative Study Report: Method AOAC 2011.06 (Folate by LC-MS/MS) 40