AOAC SPIFAN Nutrients ERP-Final Review (Oct. 2018) Pubs

2011.06 (September 2017)-Fol-22 MLT Report

FOR ERP USE ONLY DO NOT DISTRIBUTE

Autosampler Temperature: 5 ° C

Injection Solvent: Std., samples and blanks injection solvent is 90% MeOH + 10% Formic acid + 1% ascorbic acid neutralized to pH 7 with ammonium hydroxide (28-30%), 50 uL/mL. Injection Volume = 2 uL MS Conditions for the Sciex 6500 LC-MS/MS (Qtrap): MS conditions Sciex 6500 for detection of different transitions of the analytes in the scope Comp ound Q1 Q3 DP EP CXP CE

442.2

295.2

75

6

6

20

FA

442.2

176.2

75

6

6

45

460.2

313.2

75

6

6

28

5-CH3-THF

460.2

194.2

75

6

6

46

474.2

327.2

75

6

6

25

5-CHO-THF

474.2

299.2

75

6

6

40

13 C 5

-FA

447.2

295.2

75

6

6

20

13 C 5

-5-CH3-THF 465.2

313.2

75

6

6

28

13 C 5

-5-CHO- THF

75

6

6

479.2

327.2

25

DP: Declustering potential; EP Entrance potential; CEP collision cell entrance potential; CE Collision cell; CXP collision cell exit potential. The General MS parameters for the Sciex 6500 LC-MS/MS (Qtrap):

Collaborative Study Report: Method AOAC 2011.06 (Folate by LC-MS/MS) 41