AOAC SPIFAN Review of GB Methods (March 12, 2020)

FOR EXPERT REVIEW PANEL USE ONLY NOT FOR DISTRIBUTION

Expert Review Panel for Infant Formula and Adult Nutrition Evaluation of Method GB 5413. 18-2010

Title: Determination of vitamin C in foods for infants and young children, milk and milk products Author: National Standard of the People´s Republic of China

Reviewer Name: Reviewer 1

Summary of Method: Vitamin C (ascorbic acid) is oxidized to be dehydroascorbic acid in presence of activated carbon. The dehydroascorbic acid then reacts with o-phenylenediamine to generate fluorescent substances. The fluorescent spectrophotometer then is applied to determine the fluorescence intensity of the fluorescent substances generated. The fluorescence intensity is proportional to the concentration of ascorbic acid present.

Method Scope/Applicability: This method is applicable to determination of vitamin C in infant foods and young children, milk and milk products.

Method Clarity: The method is clear, descriptive and easy to follow in its current format.

Pros/Strengths:

Cons/Weaknesses This method could present interferences.

Supporting Data • General Comment:

Method Optimization: No data presented.

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