Amino-01 (July 2018)

Single Laboratory Validation Report for Total Amino Acids by UHPLC-UV in Infant Formulas and Adult Nutritionals

Glu Gly His Leu Lys Met Phe Pro Ser Tau Thr Tyr Val Ile

pmol/µL pmol/µL pmol/µL pmol/µL pmol/µL pmol/µL pmol/µL pmol/µL pmol/µL pmol/µL pmol/µL pmol/µL pmol/µL pmol/µL

0.25 0.50 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25

50 50 50 50 50 50 50 50 50 50 50 50 50 50

0.916 0.927 0.956 1.005 0.983 1.597 0.979 0.993 0.897 0.945 0.931 0.968 0.991 0.981

N N N N N N N N N N N N N N

0.087 -0.097 -0.176 -0.126 -0.145 -0.071 -0.168 -0.143 -0.066 -0.100 -0.109 -0.104 -0.145 -0.267

Y Y Y Y Y Y Y Y Y Y Y Y Y Y

0.999 0.999 0.999 0.999 0.999 0.999 0.999 0.999 0.999 0.999 0.999 0.999 0.999 0.999

0.560 0.548 0.539 0.546 0.537 0.951 0.541 0.552 0.565 0.508 0.587 0.524 0.554

0.542 (*) Cystine follows a linear regression up to 10 pmol/µL. In the matrices used for the validation of this method, concentrations of cystine did not exceed 3 pmol/µL.

Analyte

Plot of the regression line

Plot of the residuals

Ala

Arg

Jaudzems, Guthrie, Lahrichi, and Fuerer – January 2018

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