Amino-01 (July 2018)

Amino-01 (JULY 2018) 180124 AA SPIFAN SLV Appendices A-I

FOR ERP USE ONLY DO NOT DISTRIBUTE

Single Laboratory Validation Report for Total Amino Acids by UHPLC-UV in Infant Formulas and Adult Nutritionals -– APPENDICES (A to I) Appendix A : average values of the SPIFAN II SLV kit products in mg/100 g reconstituted product.

SPIFAN II SLV kit product

Analyte

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

16

17

18

19

His

41.2

45.3 126.5 195.8

27.0

34.6

22.2

31.9

35.5

27.2

44.9

37.9

44.2

28.0

25.6

33.6

28.0 129.0 189.0

Tau Ser Arg Gly

N/A

N/A

N/A

N/A

N/A

4.1

4.7

5.2

3.5

4.7 N/A

8.1

4.0

3.8

4.7

6.4

4.7

17.5

20.1

98.2

62.3 307.8 448.8

82.3 35.8 28.0

78.4 43.3 27.0

71.6 29.8 24.7

73.3 94.4 56.5

87.5 46.3 29.9

65.9 35.2

71.0 87.2

88.9

57.9

66.8 36.8 25.2

66.3

75.2

79.3 312.0 461.8 36.0 707.5 237.0 28.0 106.3 141.0

63.6 105.4 709.3 244.0

59.3 103.8

34.1 100.7

36.3

47.3 106.0 141.8

26.6 153.3

35.4

48.8

22.5

58.3

Asp 158.1 198.1 503.0 581.3 155.8 126.2 159.4 173.4 147.9 121.4 143.5 147.1 195.9 127.3 113.9 173.5 145.8 491.8 583.3 Glu 363.5 244.0 1237.8 1789.0 302.5 305.4 241.5 279.1 334.3 209.8 243.4 334.9 239.9 248.5 244.4 281.4 287.8 1205.8 1785.5 Thr 79.6 79.7 252.5 326.5 87.0 70.3 93.1 52.8 79.1 61.1 69.5 75.4 78.9 65.7 66.3 54.0 85.8 252.8 328.5 Ala 64.3 64.6 218.8 240.8 65.3 52.2 69.5 61.9 61.3 40.6 89.5 60.4 62.5 53.1 49.7 61.5 62.3 214.8 239.5 Pro 146.4 61.4 519.0 840.5 116.3 129.8 77.6 70.4 134.7 79.0 129.2 136.2 60.8 93.1 97.0 72.1 113.5 510.8 835.8 Lys 143.4 119.8 491.0 594.8 121.0 120.8 131.6 89.3 141.4 100.4 98.0 132.0 111.6 112.6 100.2 87.5 115.5 484.8 597.8 Tyr 75.6 93.2 267.5 398.0 55.8 64.3 37.1 49.7 68.7 57.3 69.1 72.1 95.6 52.7 49.9 52.4 56.3 271.3 403.0 xCys 17.7 22.4 59.3 26.9 23.3 14.6 33.5 16.4 20.4 24.9 16.8 17.6 23.9 23.5 18.4 16.1 22.8 56.5 25.3 Met 38.1 41.3 138.8 196.3 31.5 51.0 25.6 34.1 36.6 22.3 37.3 36.0 43.0 29.6 28.6 41.4 32.0 141.5 199.5 Val 96.9 141.4 326.0 489.4 81.8 90.8 69.0 56.5 88.9 64.9 98.4 90.7 142.5 74.1 68.8 62.3 88.3 324.0 477.8 Ile 84.6 122.8 274.3 380.9 76.8 79.7 74.4 56.0 78.5 59.2 86.8 80.5 124.6 68.8 63.4 62.6 84.8 270.0 372.3 Leu 164.6 203.3 584.3 724.8 150.3 139.9 138.3 109.0 157.5 111.6 140.6 155.5 200.7 133.3 120.6 111.3 148.8 574.5 723.0 Phe 79.2 103.1 258.8 397.3 59.3 65.5 42.0 69.0 68.9 49.4 78.0 75.5 104.9 54.7 51.1 74.1 58.8 260.5 388.3 Values are in mg/100 g reconstituted product. Those groups determine the performance requirement for each analyte/matrix pair according to the SMPR. * updated version proposed by the amino acid working group to be reviewed by the stakeholder panel during the August 2018 annual meeting.

Jaudzems, Guthrie, Lahrichi, and Fuerer – January 2018

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