Amino-01 (July 2018)

Amino-01 (JULY 2018) 180124 AA SPIFAN SLV Appendices A-I

FOR ERP USE ONLY DO NOT DISTRIBUTE

Single Laboratory Validation Report for Total Amino Acids by UHPLC-UV in Infant Formulas and Adult Nutritionals -– APPENDICES (A to I) Appendix E : reproducibility performance requirements for each analyte/sample pair according to the concentration of the analyte in the reconstituted product.

SPIFAN II SLV kit product

Analyte

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

16

17

18

19

His

8% 5% 8% 8% 8% 5% 5% 8% 8% 8% 8% 8% 8% 8% 8% 8% 5% 8%

8% 5% 8% 8% 8% 5% 5% 8% 8% 8% 8% 8% 8% 8% 8% 8% 5% 8%

8% 5% 5% 5% 8% 5% 5% 5% 5% 5% 5% 5% 8% 8% 5% 5% 5% 5%

5% 5% 5% 5% 8% 5% 5% 5% 5% 5% 5% 5% 8% 5% 5% 5% 5% 5%

8% 5% 8% 8% 8% 5% 5% 8% 8% 8% 8% 8% 8% 8% 8% 8% 5% 8%

8%

8%

8% 8% 8% 8% 8% 5% 5% 8% 8% 8% 8% 8% 8% 8% 8% 8% 8% 8%

8%

8%

8% 5% 8% 8% 5% 8% 5% 8% 8% 8% 8% 8% 8% 8% 8% 8% 8% 8%

8% 8% 8% 8% 8% 8% 5% 8% 8% 8% 8% 8% 8% 8% 8% 8% 5% 8%

8%

8%

8%

8% 8% 8% 8% 8% 5% 5% 8% 8% 8% 8% 8% 8% 8% 8% 8% 8% 8%

8%

8% 8% 5% 5% 8% 5% 5% 5% 5% 5% 5% 5% 8% 8% 5% 5% 5% 5%

5% 8% 5% 5% 8% 5% 5% 5% 5% 5% 5% 5% 8% 5% 5% 5% 5% 5%

Tau Ser Arg Gly Asp Glu Thr Ala Pro Lys Tyr

11%

11%

11%

11%

11%

11%

11%

11%

8% 8% 8% 8% 5% 8% 8% 8% 8% 8% 8% 8% 8% 8% 8% 8%

8% 8% 8% 5% 5% 8% 8% 8% 8% 8% 8% 8% 8% 8% 8% 8%

8% 8% 8% 8% 5% 8% 8% 8% 8% 8% 8% 8% 8% 8% 5% 8%

8% 8% 8% 8% 5% 8% 8% 8% 8% 8% 8% 8% 8% 8% 8% 8%

8% 8% 8% 5% 5% 8% 8% 8% 8% 8% 8% 8% 8% 8% 5% 8%

8% 8% 8% 8% 5% 8% 8% 8% 8% 8% 8% 8% 8% 8% 8% 8%

8% 8% 8% 8% 5% 8% 8% 8% 8% 8% 8% 8% 8% 8% 8% 8%

8% 8% 8% 8% 5% 8% 8% 8% 8% 8% 8% 8% 8% 8% 8% 8%

xCys Met

Val

Ile

Leu Phe

Those reproducibility performance requirements were set for each analyte/matrix pair according to the concentration of the analyte in mg/100 g reconstituted product (Appendix A) and the SMPR*. Colors are used for clarity. * updated version proposed by the amino acid working group to be reviewed by the stakeholder panel during the August 2018 annual meeting.

Jaudzems, Guthrie, Lahrichi, and Fuerer – January 2018

page 5/22