Amino-01 (July 2018)

Amino-01 (JULY 2018) 180124 AA SPIFAN SLV Appendices A-I

FOR ERP USE ONLY DO NOT DISTRIBUTE

Single Laboratory Validation Report for Total Amino Acids by UHPLC-UV in Infant Formulas and Adult Nutritionals -– APPENDICES (A to I) High concentration standard (50 pmol/μL, 1 μL injected). Full (top) and zoomed (bottom) views

1.60

1.40

1.20

AMQ - 2.795

1.00

0.80

AU

0.60

0.40

Lys - 17.262

Deriv Peak - 16.956 17.068 17.143 Tyr - 17.630

0.20

Val - 18.291

Met - 18.018

Ile - 20.074

Leu - 20.295

Phe - 20.584

Pro - 15.728

Ala - 14.579

Thr - 13.528

NH3 - 3.284

mono-LYS - 4.555 Tau - 4.816

Glu - 12.026

Ser - 6.128

His - 3.653

Gly - 7.523

Arg - 7.060

Asp - 9.237

NVa - 18.595 18.808

21.854

20.915

21.360

19.558

16.637

16.002

16.276

12.826

14.929

1.667

0.00

Minutes 1.00 1.50 2.00 2.50 3.00 3.50 4.00 4.50 5.00 5.50 6.00 6.50 7.00 7.50 8.00 8.50 9.00 9.50 10.00 10.50 11.00 11.50 12.00 12.50 13.00 13.50 14.00 14.50 15.00 15.50 16.00 16.50 17.00 17.50 18.00 18.50 19.00 19.50 20.00 20.50 21.00 21.50 22.00

0.00 0.02 0.04 0.06 0.08 0.10 0.12 0.14 0.16 0.18 0.20 0.22 0.24 0.26 0.28 0.30 0.32 0.34 0.36 0.38

AMQ - 2.795

Lys - 17.262

Tyr - 17.630

Deriv Peak - 16.956 17.068 17.143

AU

Val - 18.291

Met - 18.018

Ile - 20.074

Leu - 20.295

Phe - 20.584

Pro - 15.728

Ala - 14.579

Thr - 13.528

NH3 - 3.284

Tau - 4.816

Glu - 12.026

Ser - 6.128

His - 3.653

Gly - 7.523

Arg - 7.060

Asp - 9.237

NVa - 18.595

20.915

21.360

19.558

mono-LYS - 4.555

18.808

16.637

16.002

16.276

14.929

12.826

1.667

Minutes 1.50 2.00 2.50 3.00 3.50 4.00 4.50 5.00 5.50 6.00 6.50 7.00 7.50 8.00 8.50 9.00 9.50 10.00 10.50 11.00 11.50 12.00 12.50 13.00 13.50 14.00 14.50 15.00 15.50 16.00 16.50 17.00 17.50 18.00 18.50 19.00 19.50 20.00 20.50 21.00 21.50

Jaudzems, Guthrie, Lahrichi, and Fuerer – January 2018

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