Amino-01 (July 2018)

Amino-01 (JULY 2018) 180124 AA SPIFAN SLV Appendices A-I

FOR ERP USE ONLY DO NOT DISTRIBUTE

Single Laboratory Validation Report for Total Amino Acids by UHPLC-UV in Infant Formulas and Adult Nutritionals -– APPENDICES (A to I) SPIFAN II SLV kit product #3: Adult Nutritional RTF, High Protein (Placebo)

0.00 0.02 0.04 0.06 0.08 0.10 0.12 0.14 0.16 0.18 0.20 0.22 0.24 0.26 0.28 0.30 0.32 0.34 0.36 0.38 0.40 0.42 0.44 0.46 0.48 0.50 0.52 0.54

AMQ - 2.803

Lys - 17.261

Leu - 20.293

Pro - 15.727

AU

Glu - 12.020

Val - 18.289

Ile - 20.074

NH3 - 3.283

Ala - 14.580

Tyr - 17.630

20.484 Phe - 20.584 20.929

Thr - 13.532

Arg - 7.054

Ser - 6.126

Deriv Peak - 16.955

Asp - 9.237

NVa - 18.594 18.669 18.875 18.979

xCys - 17.914 Met - 18.018

Gly - 7.523

His - 3.655

21966

21.582 21.629 21.688 21.754 21.794 21.847

16.637 16.741 16.827

19.713

21.184

19.843

19.365 19.418

mono-LYS - 4.556

2.482

19.128

12.864

10.782

16.495

17.069 17.146

7.970

15.841 15.899 15.951

9.746

2.127

13.174

14.815

16.140 16.226 16.272 16.338

15.572

15.048 15.078 15.135 15.202 15.258

14.290

13.937

11.147

1.308

9.951

11.284

11.508

Minutes 1.00 1.50 2.00 2.50 3.00 3.50 4.00 4.50 5.00 5.50 6.00 6.50 7.00 7.50 8.00 8.50 9.00 9.50 10.00 10.50 11.00 11.50 12.00 12.50 13.00 13.50 14.00 14.50 15.00 15.50 16.00 16.50 17.00 17.50 18.00 18.50 19.00 19.50 20.00 20.50 21.00 21.50 22.00

SPIFAN II SLV kit product #4: Adult Nutritional RTF, High Fat (Placebo)

0.250

0.240

0.230

AMQ - 2.970

0.220

Lys - 17.369

0.210

0.200

Pro - 15.909

0.190

0.180

0.170

0.160

0.150

Glu - 12.670

Leu - 20.379

0.140

0.130

Val - 18.402

0.120

AU

0.110

0.100

0.090

NH3 - 3.544

Ile - 20.163

0.080

0.070

Tyr - 17.773

Phe - 20.688

0.060

Ala - 14.845

Thr - 13.899

0.050

0.040

NVa - 18.703

Deriv Peak - 17.072

Ser - 6.819

Asp - 10.250

0.030

0.020

Gly - 8.352

21.765 21.874

His - 4.057

Arg - 8.051

0.010

xCys - 18.047 Met - 18.150

20.991

19.492

17.483

18.981

16.942

6.586

11.264

13.260

8.835

2.567

mono-LYS - 5.084

2.208

10.680

0.000

Minutes 1.00 1.50 2.00 2.50 3.00 3.50 4.00 4.50 5.00 5.50 6.00 6.50 7.00 7.50 8.00 8.50 9.00 9.50 10.00 10.50 11.00 11.50 12.00 12.50 13.00 13.50 14.00 14.50 15.00 15.50 16.00 16.50 17.00 17.50 18.00 18.50 19.00 19.50 20.00 20.50 21.00 21.50 22.00

Jaudzems, Guthrie, Lahrichi, and Fuerer – January 2018

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