Amino-01 (July 2018)

Amino-01 (JULY 2018) 180124 AA SPIFAN SLV Appendices A-I

FOR ERP USE ONLY DO NOT DISTRIBUTE

Single Laboratory Validation Report for Total Amino Acids by UHPLC-UV in Infant Formulas and Adult Nutritionals -– APPENDICES (A to I)

SPIFAN II SLV kit product #5: Infant Formula RTF, Milk Based (Placebo)

0.090

0.085

0.080

AMQ - 2.977

0.075

0.070

0.065

Lys - 17.376

0.060

0.055

0.050

Deriv Peak - 17.078

0.045

Leu - 20.389

AU

NVa - 18.712

0.040

Pro - 15.927

Glu - 12.768

0.035

Val - 18.412

0.030

NH3 - 3.573

Ile - 20.172

0.025

Ala - 14.878

Thr - 13.949

0.020

Phe - 20.701

Tyr - 17.788

0.015

21.876

Asp - 10.417

xCys - 18.061 Met - 18.163

0.010

Ser - 6.925

0.005

Gly - 8.481

17.514

20.994

18.980

His - 4.104

Arg - 8.209

16.950

11.333

6.647

2.212

13.396

8.960

0.000

Minutes 1.00 1.50 2.00 2.50 3.00 3.50 4.00 4.50 5.00 5.50 6.00 6.50 7.00 7.50 8.00 8.50 9.00 9.50 10.00 10.50 11.00 11.50 12.00 12.50 13.00 13.50 14.00 14.50 15.00 15.50 16.00 16.50 17.00 17.50 18.00 18.50 19.00 19.50 20.00 20.50 21.00 21.50 22.00

SPIFAN II SLV kit product #6: NIST SRM 1849A

0.120

0.115

0.110

AMQ - 2.962

0.105

Lys - 17.369

0.100

0.095

0.090

0.085

0.080

0.075

0.070

Pro - 15.909

0.065

Leu - 20.380

0.060

AU

Glu - 12.676

0.055

Val - 18.402

0.050

NH3 - 3.544

0.045

Deriv Peak - 17.070

0.040

NVa - 18.702

Ile - 20.163

0.035

0.030

Ala - 14.843

Tyr - 17.771

0.025

Phe - 20.688

Thr - 13.899

0.020

Asp - 10.255

0.015

21.874

Ser - 6.820

0.010

xCys - 18.045 Met - 18.149

Gly - 8.355

0.005

His - 4.055

Arg - 8.057

17.481

20.992

18.980

2.208

11.262

6.586

13.328

Tau - 5.332

8.844

0.000

Minutes 1.00 1.50 2.00 2.50 3.00 3.50 4.00 4.50 5.00 5.50 6.00 6.50 7.00 7.50 8.00 8.50 9.00 9.50 10.00 10.50 11.00 11.50 12.00 12.50 13.00 13.50 14.00 14.50 15.00 15.50 16.00 16.50 17.00 17.50 18.00 18.50 19.00 19.50 20.00 20.50 21.00 21.50 22.00

Jaudzems, Guthrie, Lahrichi, and Fuerer – January 2018

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