Amino-01 (July 2018)
Amino-01 (JULY 2018) 180124 AA SPIFAN SLV Appendix J
FOR ERP USE ONLY DO NOT DISTRIBUTE
Single Laboratory Validation Report for Total Amino Acids by UHPLC-UV in Infant Formulas and Adult Nutritionals – APPENDIX J
ERP request: investigate optimizing chromatography (peak resolution criteria) cysteine methionine This has not been a request for other SPIFAN methods. If specific criteria (USP or other) is required, laboratories would not have the ability to reference their own quality system criteria. Below is a typical expectation of CYS-MET resolution. This is zoomed closer than provided in the original SLV report. Note the USP resolution of 1.6. Method authors do not recommend adding this criteria to the method.
NIST 1849a
0.065
0.060
0.055
Val - 18.434
0.050
0.045
0.040
NVa - 18.738
0.035
AU
Tyr - 17.790
0.030
Met - 18.175
0.025
0.020
0.015
0.010
xCys - 18.068
0.005
0.000
Minutes 17.55 17.60 17.65 17.70 17.75 17.80 17.85 17.90 17.95 18.00 18.05 18.10 18.15 18.20 18.25 18.30 18.35 18.40 18.45 18.50 18.55 18.60 18.65 18.70 18.75 18.80 18.85 18.90
Jaudzems, Guthrie, Lahrichi, and Fuerer – January 2018
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