ERP Micro December 2019

Evaluation of the GENE-UP ® Listeria spp. Method for the Detection of Listeria Species in a Variety of Foods and Select Environmental Surfaces: Collaborative Study

Ronald Johnson & John Mills bioMérieux, Inc. 595 Anglum Road, Hazelwood, MO, 63042 USA

Jean-Louis Pittet bioMérieux, Inc. Chemin de l’Orme, 69280 Marcy L’Etoile France

Olivier Mathia Institut Scientifique d’Hygiène & d’Analyse, 17 rue Doyen Denis Leroy F-35000 Rennes France

Patrick Bird & Maria Nelson AOAC International, 2275 Research Blvd. #300, Rockville, MD, 20850 USA

Background: The GENE-UP ® Listeria spp. 2 (LIS 2) assay ( Performance Tested Method SM 121803) is a real-time PCR molecular detection method for the rapid detection of Listeria species (including Listeria monocytogenes ) in a variety of foods and environmental surfaces. Objective: The purpose of this validation was to evaluate the method’s interlaboratory performance and submit the result to AOAC INTERNATIONAL for adoption as First Action Official Method SM for the detection of Listeria species in a variety of foods and select environmental

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