ERP Micro December 2019

Table 2019.2A (cont’d.) . Comparative results for the Detection of Listeria species in full-cream goat milk cottage cheese (8.4% fat) test portions by the 1 GENE-UP ® Listeria spp. 2 (LIS 2) method vs. ISO 11290-1/Amd.1 in a collaborative study (low inoculum level) 2

C vs. R CP vs. CC

Candidate presumptive (CP) Candidate confirmed (CC)

Candidate result (C)

Reference method (R)

Statistic

Matrix

Laboratory

POD (CP) 1.00 1.00 1.00 1.00 1.00 1.00 0.63 0.25 0.13 0.50 N/A 0.75 0.88 1.00 1.00 0.38 0.77 0.60 0.94 0.32 0.28 0.37 0.29 0.20 0.48 0.43 0.37 0.53

POD (CC) 1.00 0.88 1.00 1.00 1.00 0.88 0.63 0.25 0.13 0.50 N/A 0.75 0.88 1.00 1.00 0.38 0.75 0.59 0.91 0.34 0.30 0.39 0.28 0.18 0.46 0.44 0.38 0.53

POD (C) 1.00 0.88 1.00 1.00 1.00 0.88 0.63 0.25 0.13 0.50 N/A 0.75 0.88 1.00 1.00 0.38 0.75 0.59 0.91 0.34 0.30 0.39 0.28 0.18 0.46 0.44 0.38 0.53

POD (R) 1.00 1.00 1.00 1.00 1.00 0.88 1.00 0.25 0.63 0.50 N/A 1.00 1.00 1.00 1.00 0.13 0.83 0.66 0.99 0.26 0.23 0.30 0.29 0.20 0.47 0.39 0.33 0.53

dLPOD (C,R)

dLPOD (CP,CC)

N

X

N

X

N

X

N

X

1 2 3 4 5 6 7 8 9

8 8 8 8 8 8 8 8 8 8 8 8 8 8 8

8 8 8 8 8 8 5 2 1 4 6 7 8 8 3

8 8 8 8 8 8 8 8 8 8 8 8 8 8 8

8 7 8 8 8 7 5 2 1 4 6 7 8 8 3

8 8 8 8 8 8 8 8 8 8 8 8 8 8 8

8 7 8 8 8 7 5 2 1 4 6 7 8 8 3

8 8 8 8 8 8 8 8 8 8 8 8 8 8 8

8 8 8 8 8 7 8 2 5 4 8 8 8 8 1

0.00 -0.12 0.00 0.00 0.00 0.00 -0.37 0.00 -0.50 0.00 -0.25 -0.12 0.00 0.00 0.25 -0.08 -0.31 0.16 N/A

0.00 0.12 0.00 0.00 0.00 0.12 0.00 0.00 0.00 0.00 N/A 0.00 0.00 0.00 0.00 0.00 0.02 -0.22 0.25

Full- Cream Goat Milk Cottage Cheese (8.4% Fat)

10

CFU/ Test Portion

N/A

N/A

N/A

N/A

N/A

N/A

N/A N/A

11 a

12 13 14 15 16

1.9 1.5 2.4

Estimate

All

120

92

120

90

120

90

120

98

LCL UCL LCL UCL LCL UCL UCL LCL s r b s L c s R d

P T e 0.0000 a Collaborator Data Not Included in Statistical Analysis, b Repeatability Standard Deviation, c Among-Laboratory Standard Deviation, d Reproducibility Standard Deviation, e P T 0.0000 0.0000 0.0000

3 4

Value = Homogeneity test of laboratory PODs

N - Number of Test Portions; X – Number of Positive Test Portions; LCL – Lower Confidence Limit; UCL – Upper Confidence Limit

30

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