ERP Sulfite Methods-Meeting Materials_4-2020

ERP for Sulfites in Seafood Methods Materials

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Biolan Microbiosensores BIOFISH 300 SUL Collaborative Study Protocol

February 2019

KITMFSUL/SEFSUL: According to Regulation (EC) No. 1272/2008:

Hazard Statements : H319. Eye Irrit.2. May cause serious eye irritation. Precautionary Statements :

P264 Wash thoroughly after handling. P280 Wear eye protection/face protection. P305+P351+P338 In case of eye contact: rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. P337+P313 If eye irritation persists, get medical advice. Test Portion Analysis On Wednesday, all collaborators shall initiate the extraction and analysis of the Control Samples and blind coded test portions according to the BIOFISH 300 SUL method (Appendix 3.3). A copy of the detailed method will be provided for you. It is important that all collaborators perform the study on the same day and complete all the testing within one day. All test portions are pre ‐ weighed. Each collaborator will analyze their test portions as follows. 1. Refer to the Test Portion Inventory Sheet for the BIOFISH 300 SUL range to use for each test portion. There are 4 blind coded test portions for each method range. Group the test portions by range keeping them frozen. 2. Starting with the Control Samples and test portions for the first range, 7 ‐ 30 mg/kg, allow them to sit at room temperature for 15 ‐ 20 minutes to thaw, with the plastic tube closed, as it was received. 3. Add 18 mL of SUL extraction solution to each test portion. 4. Homogenize the contents with the aid of an UltraTurrax set at 8000 ‐ 9000 rpm for 15 ‐ 20 sec, ensuring that all the sample is mixed with the liquid. 5. Let stand a minimum of 15 minutes at room temperature to complete the extraction. Once extracted, the samples are stable up to 1 h at room temperature. 6. Follow the BIOFISH 300 SUL 7 ‐ 30 mg/kg method to set up the device (recondition, activation and calibration). 7. Follow the BIOFISH 300 SUL 7 ‐ 30 mg/kg method to complete the analyses of the Control Samples. Analyze two aliquots of the extract for each Control Sample (total of 4 analyses). 8. Verify that the Negative Control Sample gives a mean result of 0 mg/kg, and that the Positive Control Sample gives a mean result of X ± Y mg/kg (range to be provided once known). If results are in range, proceed to the next step. If results are not in range, contact the Study Director. 9. Follow the BIOFISH 300 SUL 7 ‐ 30 mg/kg method to complete the analyses of the blind coded test portions. Only one analysis is required for each extract. Make sure to conduct the analysis within 1 hour of extraction. 10. Follow the method to complete the analysis for each blind coded test portion in the remaining 2 ranges. 11. Record all results on the Data Report Form (Appendix 3.5).

DRAFT

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04/16/2020