Food Authenticity Program Meeting Book (March 13, 2020)

Vertebrate Genomes

Tetraodon nigroviridis (Green Spotted Puffer) Protopterus aethiopicus (Marbled Lungfish)

340 Million bp

Homo sapiens 3.2 Billion bp

130 Billion bp

“DNA Barcode” – 655 bp (1/1,000,000)

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DNA Barcoding Workflow

1. Tissue Sampling

2. Tissue Lysis and DNA Extraction

3. Polymerase Chain Reaction – COI Amplification

4. PCR Cleanup

5. Cycle Sequencing Reaction

6. Sequencing Reaction Cleanup

7. Sequencing

8. Post Sequencing Analysis

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