Food Authenticity Program Meeting Book (March 13, 2020)

SANGER SEQUENCING • For Sanger sequencing identification methods, the basic performance characteristics are: – Extraction Efficiency – Quality of Sequencing Template

– Quality of Sequencing Data – Robustness/Ruggedness – Precision – Specificity – Accuracy – Confirmation of Identity

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REFERENCE MATERIALS • Reference materials refers to specimens which can be used to establish species identity – Optimally, reference sequences should be generated under a defined set of conditions to assure quality – However, some reference sequences can come from public databases • It is incumbent on the originating laboratory to verify the validity and quality of the sequence used • Accession numbers used for the development of molecular assays (and any supporting documentation) should be included in the validation package

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