Food Authenticity Program Meeting Book (March 13, 2020)

METHOD VALIDATION DOCUMENTS

For Qualitative and Quantitative PCR-Based Assays: Assay Design • Type of assay: oligonucleotide probe-based or double stranded DNA dye-based • Name of target gene or region • Internal control/amplification control type: exogenous or endogenous • Exact oligonucleotide sequences for all primers and probe(s) • Length of PCR product (amplicon) • Dye and probe characteristics. (Dyes used and probe chemistries etc.).

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METHOD VALIDATION DOCUMENTS CONT’D

Sample Preparation and nucleic acid Extraction • Form and quantity of sample required. Include information on subsampling or sample compositing as well as relevant aspects of handling and storage. • Method or kit used for DNA extraction. Include any relevant modifications as well as information on RNAse treatment.

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