Food Authenticity Program Meeting Book (March 13, 2020)

Definitions Single Laboratory Validation (SLV) —

" Guidelines on the use of Mass Spectrometry (MS) for Identification, Confirmation and Quantitative analysis of Residues CAC/GL 56-2005 "; " Establishing the Fitness for Purpose of Mass Spectrometric methods." Bethem, R., Boison, J.O., Gale, J., Heller, D., Lehotay, S., Loo, J., Musser, S., Price, P., and Stein, S. (2003). Journal of the American Society for Mass Spectrometry 14: 528-541. SANTE/12682/2019. "Method Validation and Quality Control Procedures for Pesticide Residues Analysis in Food and Feed“ - A Guidance document on analytical quality control and method validation procedures for pesticide residues analysis in food and feed. Once the method has been demonstrated to meet the minimum requirements for validation and fit for purpose criteria, the method can be reviewed and considered by AOACI for classification as First Action Official Method of Analysis.

Table A2: Expected Precision as a Function of Concentration-Repeatability

Analyte (%)

Analyte Ratio

Unit (%, mg/kg)

%RSD

% Mean Recovery

100

1.0

100

1.3 1.9 2.7 3.7 5.3 7.3

98-102 98-102 97-103 95-105 90-107 80-110 80-110 80-110 60-115 40-120

10

10 -1 10 -2 10 -3 10 -4 10 -5 10 -6 10 -7 10 -8 10 -9

10

1

1

0.01

0.1

0.001

100 mg/kg (ppm) 10 mg/kg (ppm) 1 mg/kg (ppm) 100 g/kg (ppb) 10 g/kg (ppb) 1 g/kg (1ppb)

0.0001 0.00001 0.000001 0.0000001 0.00000001

11 15 21 30

8