ISPAM Stakeholder Panel Meeting Book 3-14-17

• [1] Official Methods of Analysis of AOAC INTERNATIONAL (2012) 19th Ed., AOAC INTERNATIONAL, Gaithersburg, MD, USA, APPENDIX I; also on-line at http://www.eoma.aoac.or

Identification of Venezuelan Equine Encephalitis Virus (VEEV)

Parameter

Minimum Performance Requirement

AMIL

5000 genome copies / mL

POI at AMIL within sample collection buffer POI at AMIL in an aerosol environmental matrix System False-Negative Rate using spiked aerosol environmental matrix System False-Positive Rate using aerosol environmental matrix

•

• 0.95

” %

” %

Inclusivity panel purified DNA All inclusivity strains (Table II) must be correctly identified at 2x the AMIL † Exclusivity panel purified DNA All exclusivity strains (Table III and Annex IV; part 2) must test negative at 10x the AMIL †

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Table II: Inclusivity Panel

• [1] Official Methods of Analysis of AOAC INTERNATIONAL (2012) 19th Ed., AOAC INTERNATIONAL, Gaithersburg, MD, USA, APPENDIX I; also on-line at http://www.eoma.aoac.or

Identification of Venezuelan Equine Encephalitis Virus (VEEV)

Inclusivity Panel

Representative Strain (s) Trinidad Donkey MF-8 ICVE93, ICVE95 IEMX63, IEPA62 1DPA61, 1DPE98, IDPE06

VIRUS

Serotype / Variant

VEE-IAB VEE-IC

VEEV

VEE-IE

VEE-ID

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