ISPAM Stakeholder Panel Meeting Book 3-14-17

Background and positioning of Working Group

‰ Working Group reporting to the AOAC International Stakeholder Panel on Alternative Methodologies (ISPAM) to improve food allergen methods.

‰ WG tasked specifically to develop recommendations for the establishment of Standard Method Performance Requirements (SMPR) for food allergen methodologies ‰ WG creation follows a priority setting exercise for work on Food Allergens SMPR – Thought Leader Advisory Meeting on June 15 th at AOAC HQ.

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Background on Food Allergens Methods

™ Most Methods are Antibody based methods: • ELISA tend to dominate existing methodologies being applied for food control purposes • Other detection approaches are possible e.g. SPR-based biosensors ™ Some methods are DNA/RNA based methods – used generally as complementary methods (particularly in jurisdictions where the regulations are based on protein requirements) ™ Methods based on Mass-Spectrometry detection (with the associated separation technique) are confirmatory approaches.

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