ISPAM Stakeholder Panel Meeting Book 3-14-17

SMPR CHALLENGES - RECOVERY

• Accuracy/ “Trueness” in gluten analyses has always been a difficult concept to formally define and experimentally estimate. • In the past, barley has always been a minor consideration for choosing antibodies. • How to develop criteria to evaluate relative responses of wheat, rye and barley? • Criteria for kit response (extraction + antibody) should be evaluated individually for 3 grains.

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GLUTEN R5 Assay Kit Relative Response by Spiked Grain Weight

70.00

y = 0.2965x + 0.2085 R² = 0.9977

60.00

BARLEY SLOPE = 29.7%

50.00

y = 0.2362x + 2.4442 R² = 0.9893

40.00

RYE SLOPE = 23.6%

30.00

GLUTEN MG/KG

20.00

WHEAT SLOPE = 10.3%

y = 0.1034x + 0.8348 R² = 0.999

10.00

0.00

0

50

100

150

200

SPIKE MG/KG

Barley

Rye

Wheat

Linear (Barley)

Linear (Rye)

Linear (Wheat)

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