Micro Community Meeting Book

Amplicon Sequencing Minimal Information (AsqMI): Quality and Reporting Guidelines for Actionable Calls in Biodefense Applications

• Amplicon sequencing (AS) is a good biosurveillance tool compared to quantitative polymerase chain reaction for routine biosurveillance of biothreat agents. • The added specificity provide by sequencing the amplicon leads to fewer false negative and false positive calls. • Appropriate controls and workflow reporting are necessary to address difference in sequencing platforms, the approaches used to amplify and sequence the target and the algorithms associated with base calling, i.e., Fourier transform or neural network (AI).