Micro Community Meeting Book

 Use for analysis of aerosol collection dry filter unit (DFU) filters as part of concept of operation-based routine testing to make actionable calls. Standard Method Performance Requirements (SMPRs  ) for Detection of Multiple Biothreat Agent Organisms in Environmental Samples by Amplicon Sequencing  Simultaneous detection of Bacillus anthracis , Francisella tularensis , Yersinia pestis , Burkholderia mallei , Burkholderia pseudomallei , Brucella abortus , Coxiella burnetii , and Variola virus from DFU filters or polytetrafluoroethylene (PTFE) filters.

 A suitable method can target some or all of these agents as appropriate for the specific biodefense program or concept of operations.

 New sequencing tools can provide a metagenomic non-targeted approach, but crically removing false positives will be more important