Microbiology Methods for ERP Review 3-2020

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The Stop Solution contains sulfuric acid which is corrosive. Wash immediately with large quantities of water if the solution comes into contact with skin or mucous membranes. All other Solus One Salmonella kit components are non-hazardous. However, product usage should follow good laboratory practices and all disposable materials must be discarded according to appropriate waste procedures used in the laboratory. Protective clothing should be worn including laboratory coat, safety glasses and gloves where appropriate. Avoid contact with the skin. Improper use of the Dynex DS2 may cause personal injury or damage to the instrument. For safe use, the Dynex DS2 must be operated only by qualified laboratory personnel who have been appropriately trained. Salmonella is a Biosafety Level 2 organism. Biological samples such as enrichments have the potential to transmit infectious diseases. Follow all applicable local, state/provincial, and/or national regulations on disposal of biological wastes. Wear appropriate protective equipment which includes but is not limited to protective eyewear, face shield, clothing/lab coat, and gloves. All work should be conducted in properly equipped facilities utilizing the appropriate safety equipment (for example, physical containment devices). Individuals should be trained in accordance with applicable regulatory and company/institution requirements before working with potentially infectious materials. When testing is complete, all materials and media possibly containing pathogens should be decontaminated follow current industry standards for the disposal of contaminated waste (i.e. autoclave for 20 min at 121 o C). Consult the Safety Data Sheet for additional information and local regulations for disposal. Enrichment

D. Sample Enrichment

Instant Non-Fat Dry Milk (a) (a)To each 375 g, homogenize (2 min) in 1125 mL of supplemented BPW (pre- warmed to 37°C) and incubate portions at 41.5 ± 1°C for 21–22 hours.

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