Microsoft Word - Draft OMB Meeting Agenda-April 11 2019

OMB Meeting 4-11-2019 Pre-read Materials

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AOAC RESEARCH INSTITUTE Expert Review Panel Chair Report for Microbiology for Foods and Environmental Surfaces Page 7 of 7

B. Since the samples are spiked (no issues typically finding the organism) is it acceptable to remove the extended confirmation for the collaborative study (transfer of test kit primary enrichment to reference secondary enrichment and subsequent confirmation)? All PTM and preliminary matrix evaluations were all tested using the extended confirmation. i. No, it is not acceptable. C. If the test kit enrichment is incubated at 41.5C in the collaborative study (EU temps), is it acceptable to make the claim for 42C in the US? Both have a +/- 1C range. i. Yes, it is acceptable. D. Another temperature example is for the selective agar plates and additional confirmation media required by the MLG. Can 37C (EU temp) be used and still be acceptable (as MLG states 35 +/- 1C)? I know ISPAM approved equivalence of 35 and 37 for Salmonella primary enrichments. i. 36 ± 2C E. For confirmation, is it acceptable to select one colony from one agar plate (per enrichment) to follow through to full ID? Once again, since the samples are spiked, we are most likely going to find the target organism without more than one attempt. i. Yes, as long as it is confirmed.

IV. Action Items A.

AOAC Staff will follow-up with the USDA, ERP and Statistics Committee regarding the number of replicates and the use of 8 laboratories instead of 12.

V. Adjournment: Meeting concluded at 5:00pm.

ERP Use Only

04/05/2019