OMA Protocol Review: OMAMAN-56 Sulfites in Shrimp

100 150 200 250 300

Monier-Williams Biofish Low Range

0 50

Sample 2 Sample 3 Sample 4 Sample 5

Biofish Results (mg/kg)

OMA results (mg/kg)

Figure 14. Correlation plot of Biofish method (low range) vs Monier-Williams method. Linear regression equation: Result Biofish =Result OMA *0.9203+32.4114; R=0.9928

100 150 200 250 300

Monier-Williams Biofish High Range

0 50

Sample 2 Sample 3 Sample 4 Sample 5 Biofish Results (mg/kg) OMA results (mg/kg)

Figure 15. Correlation plot of Biofish method (high range) vs Monier-Williams method. Linear regression equation: Result Biofish =Result OMA *1.0515+16.4368; R=0.9961

Table 21. LOD and LOQ estimates from the independent lab data X 0 S b m

LOD

LOQ

Low range method (30-150 mg/kg) High range method (50-300 mg/kg)

0

1.53

0.01

5.13

15.39

0

1.98

0.01

6.64

19.92

Discussion

The aim of the present work was to receive PTM certification for the Biofish-300 SUL method for the detection and quantification of sulfites in shrimp. This technology is highly specific and selective and allows quantification of sulfites in raw shrimp head on, raw shrimp head off, and boiled shrimp. Sulfite in all the studied matrixes was extracted in an extraction solution in a short time (15-20 seconds).

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