OMA Protocol Review: OMAMAN-56 Sulfites in Shrimp

3. Tailored preparation

a) Biotest storage and hydration The Biotests are dispatched at room temperature and vacuum packed with silica gel crystals to protect them from atmospheric humidity. Once delivered, store unopened between 3-8ºC until expiration date. To hydrate the Biotest prior to using (this process should be carried out at least 24 hours prior to use), remove the silica gel crystals from inside the tube and fill it with Measurement Solution until the base of the Biotest is submerged and add 20µl of SUL Calibration Reagent ( S ee Table 2 and Table 4 ). Once hydrated, the Biotest must be stored vertically at a temperature between 3-8ºC for a maximum of 15 days.

b) Reagent Preparation • SUL Measurement Solution

SUL1 Measurement Kit and SUL2 Measurement Kit contain 8 and 5 tubes respectively to prepare the SUL Measurement Solution ( See Table 2), which are needed to hydratate the biotest and to carry out the measurements.

Table 2 . Measurement Solution Preparation.

Dissolve the content of 1 MFSUL2 in 1L of distilled water. SUL 2 Measurement Solution

SUL 1 Measurement Solution Dissolve the content of 1 MFSUL1 in 250ml of distilled water

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