OMA Protocol Review: OMAMAN-56 Sulfites in Shrimp

8. Annex

The SUL Verification Kit supplies 4 tubes of Verifying Reactive for SUL (VFSUL). This reactive will be used for the preparation of the Verifying Solution, which is recommended to analyze after the calibration process to verify that the Calibration Standard is properly prepared. Table 7 summarises both solutions preparation as well as their stability.

Table 7. Verif ying Reactive and Veryfying Solution preparation .

Verifying Solution 250 µ l Verifying Reactive + 99,75 ml of distilled water.

Verifying Reactive

Add 5ml of Extraction Solution to 1 tube of Verifying Reactive

r.t.*/ **/***

Dry Hydrated

3-8ºC/**/15 days

3-8ºC/**/15 days

*r.t. = room temperature * *d . d . = keep in a dry and dark place *** until expiration date

To analyze the Verification Solution: 1. Press Verifying Sol. and place the tray correctly with 10 ml of Measurement Solution. Press CONTINUE to start. 2. Wait until the device records the measurement target and it emits an acoustic signal. Press CONTINUE and inject the Verififying Solution into the measurement tray, with the aid of a micropipette, and wait until the process ends ( See Table 6). 3. The message ‘Solution Verified’ or ‘Incorrect solution’ will appear on the display. 4. Replace the content of the cuvette with 10 ml of new SUL Measurement Solution.

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