OMA Protocol Review: OMAMAN-56 Sulfites in Shrimp

Biolan Microbiosensores BIOFISH 300 SUL Collaborative Study Protocol

February 2019

Appendix 3.2

Study Materials and Apparatus Biolan Microbiosensores BIOFISH 300 SUL Collaborative Study

Materials provided by collaborator:

Apparatus (standard lab apparatus): (a) Tissue homogenizer .--UltraTurrax IKA T-25 or equivalent. (b) Pipets.-- Adjustable, capable of delivering 20-200 μL and 500-5000 μL. (c) Graduated cylinder.-- 25 mL. (d) Computer. —Windows-based PC with USB port to connect to device.

Consumables: (a) Pipet tips . (b) Distilled water.

Materials provided by Biolan Microbiosensores:

(a) BIOFISH 300 Biosensor .-- BF300-004 (b) BIOFISH .-- BTFSUL100 and BTFSULAL100 (c) SUL CALIBRATION KIT (KITCFSUL)

(d) SUL MEASUREMENT KIT (KITMFSUL1 or KITMFSUL2) (e) SUL EXTRACTION SOLUTION (SEFSUL 1 or SEFSUL2) (f) Draft OMA Method .-- (g) BIOFISH sample set .--1 set of 12 blind coded tubes per collaborator packaged in 50 mL conical tubes and frozen. (h) OMA sample set.-- 1 set of 6 blind coded tubes for select collaborators packaged in 50 mL conical tubes and frozen.

Collaborators preselected to perform the OMA 990.28 reference method will provide all necessary materials, reagents, and apparatus to perform the method.

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DRAFT