OMA Protocol Review: OMAMAN-56 Sulfites in Shrimp

Biolan Microbiosensores BIOFISH 300 SUL Collaborative Study Protocol

February 2019

Appendix 3.3

AOAC Official Method XXXX.XX Determination of Sulfites in Shrimp

BIOFISH 300 SUL First Action XXXX

[Applicable to the determination of total sulfites in the range 7-300 mg/kg, measured as SO 2 , in raw shrimp with heads (30-300 mg/kg range only), raw headless shrimp, and cooked shrimp.] Caution : Sulfites may cause allergy-like reactions including sneezing, runny nose or wheezing; or asthma symptoms in people with underlying asthma; or rarely hives or anaphylaxis. The use of personal protective equipment (PPE) including gloves, laboratory coat and protective eyewear is recommended. See Table XXXX.XXA for a summary of results of the collaborative study, supporting acceptance of the method. See Tables X-Y for detailed results of the collaborative study [ J. AOAC Int . XX , xxx (2019)]. A. Principle The BIOFISH-300 SUL method is based on an enzymatic electrochemical biosensor for the specific detection of sulfite. The test method consists of the extraction of sulfite in an aqueous based solution and the subsequent quantification by the biosensor after previous calibration. The biosensor is a system that incorporates a biochemical sensing element (the Biotest, based on a specific oxidative enzyme) in intimate contact with or in close proximity of a transducer system that relates the concentration of an analyte to a measurable signal. The addition of the analyte causes sequential redox reactions that involve a release of electrons proportional to the concentration of the analyte. Thus, these biosensors combine the specificity and the selectiveness of enzyme-analyte reactions with highly sensitive electrochemical transduction. B. Test Kit ( a ) BIOFISH 300 SUL.— Comprised of the following individual components available from Biolan Microbiosensores S.L.: ( 1 ) Biotest.— BTFSUL (30-300 mg/kg) or BTFSULAL (7-30 mg/kg) for 20, 50, 100, or 125 analyses. ( 2 ) SUL Calibration Kit.— KITCFSUL. ( 3 ) SUL Measurement Kit.— KITMFSUL1 (for up to 160 analyses) or KITMFSUL2 (for up to 450 analyses). ( 4 ) SUL Extraction Solution.— SEFSUL1 (for up to 50 analyses) or SEFSUL2 (for up to 100 analyses). C. Apparatus ( a ) BIOFISH 300 Biosensor .--BF300-004, includes biosensor, 2 reference electrodes, electrode storage solution, and 2 cuvettes. ( b ) Analytical balance.-- Capable of measurement to two decimal places (g). ( c ) Mincer.--

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