OMA Protocol Review: OMAMAN-56 Sulfites in Shrimp

Biolan Microbiosensores BIOFISH 300 SUL Collaborative Study Protocol

February 2019

Once per day, before analyzing samples, the Biotest must be reconditioned, activated, and calibrated. ( a ) Reconditioning.-- ( 1 ) Press Recondition, verify the proper placement of the cuvette with the 10 mL of SUL Measurement Solution, add sulfite standard (4 mL of SUL Standard 3 for the 7-30 mg/kg range; 2 mL of SUL Standard 1 for the 30-150 mg/kg range; or 1 mL of SUL Standard 2 for the 50-300 mg/kg range) into the measurement cuvette and press CONTINUE. ( 2 ) Wait for the equipment to emit an acoustic signal, press CONTINUE, and once again inject sulfite standard (4 mL of SUL Standard 3 for the 7-30 mg/kg range; 2 mL of SUL Standard 1 for the 30-150 mg/kg range; or 1 mL of SUL Standard 2 for the 50-300 mg/kg range) into the measurement cuvette and wait for the process to end. The message “Biotest reconditioned. Ready to activate” will appear on the screen. ( 3 ) Replace the contents of the cuvette with 10 mL of SUL Measurement Solution. ( b ) Activation.-- ( 1 ) Press activate, verify the proper placement of the cuvette and press CONTINUE to begin the activation. ( 2 ) Wait for the equipment to emit an acoustic signal, press CONTINUE, and inject sulfite standard (4 mL of SUL Standard 3 for the 7-30 mg/kg range; 2 mL of SUL Standard 1 for the 30- 150 mg/kg range; or 1 mL of SUL Standard 2 for the 50-300 mg/kg range) into the measurement cuvette and wait for the process to end. The message “Biotest activated. Ready for calibration” will appear on the screen. ( 3 ) Replace the contents of the cuvette with 10 mL of SUL Measurement Solution. ( c ) Calibration .--( 1 ) Press calibrate, verify the proper placement of the cuvette with 10 mL of SUL Measurement Solution, and press CONTINUE. ( 2 ) Wait for the equipment to emit an acoustic signal. Press CONTINUE and add sulfite standard into the measurement cuvette (4 mL of SUL Standard 3 for the 7-30 mg/kg range; 2 mL of SUL Standard 1 for the 30-150 mg/kg range; or 1 mL of SUL Standard 2 for the 50-300 mg/kg range). This is addition 1. Note : It is important to add the Calibration Standard immediately after pressing the CONTINUE button to record the current intensity correctly at the time of adding it. ( 3 ) Wait for the equipment to register the change in intensity and emit an acoustic signal again. Press CONTINUE and add sulfite standard again (4 mL of SUL Standard 3 for the 7-30 mg/kg range; 2 mL of SUL Standard 1 for the 30-150 mg/kg range; or 1 mL of SUL Standard 2 for the 50-300 mg/kg range). This is addition 2. ( 4 ) Wait until the parameters of the calibration appear on the screen together with a message of “calibration OK” or “calibrate again”. ( 5 ) Replace the contents of the cuvette with 10 mL of new SUL Measurement Solution. If the regression is not valid (“calibrate again” appears), change the measurement cuvette, put it on Stand by for 30 seconds and calibrate again. If the regression is valid (“calibration OK” appears), continue to Standard Measurement. ( d ) Standard measurement.-- The calibration status should be tested using the STANDARD option after every 10-15 measurements or after the device has not been running for 30 minutes. ( 1 ) Press Standard in the analysis option, check that the cuvette is correctly fitted and filled with SUL Measurement Solution and press CONTINUE to begin the Standard measurement. ( 2 ) Wait until the device records the measurement target and it issues a sound signal. Press CONTINUE and add the Calibration Standard (4 mL of SUL Standard 3 for the 7-30 mg/kg range; 2 mL of SUL Standard 1 for the 30-150 mg/kg range; or 1 mL of SUL Standard 2 for the 50-300 mg/kg range) into the measuring cuvette. ( 3 ) Wait until the “Biosensor calibrated” or “Biosensor recalibrate” message appears. (4) Replace the contents of the cuvette with 10 mL of new SUL Measurement Solution. If “Biosensor calibrated” message appeared, proceed to Determination . If “Biosensor recalibrate” message appeared, return to Step J ( c ) Calibration .

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