OMA Protocol Review: OMAMAN-56 Sulfites in Shrimp

Biolan Microbiosensores BIOFISH 300 SUL Collaborative Study Protocol

February 2019

K. Determination ( a ) Press the option ‘Turrax’ (for 30-150 or 50-300 mg/kg range) or the option ‘Sample’ (for 7-30 mg/kg range). Check that the cuvette is correctly fitted and filled with 10 ml of SUL Measurement Solution and press CONTINUE to begin the sample measurement. ( b ) Wait until the device records the measurement target and it issues an accoustic signal. Press CONTINUE and pipette the clear supernatant of the extracted sample (4 mL for the 7-30 mg/kg range; 2 mL for the 30-150 mg/kg range; or 1 mL for 50-300 mg/kg range) into the measuring cuvette. ( c ) Wait until the process ends and the device shows the result of the analysis. ( d ) Replace the contents of the cuvette with 10 mL of new SUL Measurement Solution. L. Interpretation and Test Result Report ( a ) The Biosensor expresses the analysis result in units of mg of sulfite (measured as SO 2 ) per kg of shrimp, taking into account the dilution involved in the extraction of the sample. ( b ) To save the result of an analysis, press SAVE and enter a code. ( c ) To view the saved results, enter the RECORDS option of the main menu screen . Saved results can be downloaded to a computer. ( d ) To delete the saved results, go to RECORDS and press DELETE ALL.

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DRAFT