OMA Protocol Review: OMAMAN-56 Sulfites in Shrimp

case its measurement is required, only for the 30-150 and 50-300 mg/kg range) from 15-20 shrimp.

Homogenize the bulk sample with the aid of a mincer. Add 2 g of the homogenized sample to a plastic

tube.

( b ) Sample Extraction.— Add 18 mL of SUL Extraction Solution. Homogenize the contents of the plastic tube with the aid of an ultra-turrax (8000-9000 rpm 15-20 seconds). Leave to stand for at least 15

minutes at room temperature. Sample extractions are stable up to 1 hour at room temperature.

Biosensor Setup ( a ) For low range (7-30 mg/kg) testing, choose the Biotest BTFSULAL. For medium (30-150 mg/kg) and high (50-300 mg/kg) range testing, choose the Biotest BTFSUL. Ensure Biotest is prepared as in

Electrode Hydration and Storage .

( b ) Insert the Biotest and reference electrodes into their respective connectors (place the Biotest into the carrier, tightening it with the screw), ensuring that they are well connected. For safety reasons,

it is recommended to never disconnect the reference electrode. When the equipment is disassembled

after analyses are completed, the reference electrode remains protected with the Storage Solution

supplied (4 M KCl).

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