OMA Protocol Review: OMAMAN-56 Sulfites in Shrimp

Extraction Solution] as the spiking solution and 10 µL, 20 µL, 30 µL, 40 µL, or 50 µL were added to each 2-

g test portion. The BIOFISH 300 SUL method was then performed on blinded 2-g test portions. Five

replicates per matrix per spiked level were tested each day.

( b ) Results from incurred shrimp.-- Accuracy, bias, repeatability and intermediate reproducibility results from incurred samples are presented in Tables 2 and 3 and Figure 7 shows the regression analysis

for each matrix. Accuracy was assessed as a comparison of the BIOFISH 300 SUL results with the OMA

results and ranged from 88 to 109% for values between 10 and 30 mg/kg in both raw and cooked shrimp.

For values lower than 10 mg/kg no conclusions can be drawn about the accuracy since those samples are

values lower than 10% in

below the current limit of quantification of the reference method. Even so, RSD r

all cases demonstrate the acceptable repeatability precision of BIOFISH 300 SUL at these low

concentrations. In addition, the intermediate reproducibility of the method is also verified by the similarity

of the results of each sample on two different days of analysis with two different devices and technicians

). Regression analyses of both methods showed a good

(values ranging from 3.63% to 9.09% for RSD i

correlation between the BIOFISH 300 SUL and OMA results with R 2 > 0.99 in both cases, as shown in Figure

7.

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