OMA Protocol Review: OMAMAN-56 Sulfites in Shrimp

Biolan Microbiosensores BIOFISH 300 SUL Collaborative Study Protocol

February 2019

2.4 Study schedule 2.4.1 Instruments and test kits will be provided to collaborators in the weeks before the study begins. Training will be provided at a date and time convenient for both parties. Fortified or incurred shrimp samples different from the collaborative study materials can and should be prepared and used as training samples to demonstrate proficiency. 2.4.2 Study test portions will be shipped on a Monday by overnight courier to each test site for arrival on a Tuesday. The specific dates will be determined once collaborators are confirmed. 2.4.3 All collaborators will begin analyses on that Wednesday. 2.4.4 Data forms will be completed and emailed to the Study Director within 1 week of initiation of analyses. Table 2. Study Schedule (Specific Dates TBD)

Receipt of BIOFISH 300 SUL

Submission of Data Report Form Week 2, Wednesday

Collaborator Activity

Receipt of Test Portions

Initiation of Analyses Week 1, Wednesday

Training

Week 1, Tuesday

Date

TBD

TBD

2.5

Test Portion Preparation

2.5.1 Obtain a sufficient quantity of incurred raw shrimp with heads on, raw shrimp with heads off, and boiled shrimp at the approximate sulfite concentrations in Table 1. 2.5.2 Homogenize each bulk material with a mincer and weigh 2-g test portions into 50 mL screw cap tubes for the Biofish methods. 2.5.3 For each collaborator, randomize and blind code two test portions of each material. 2.5.4 Bag and label the Biofish test portion set for each collaborator. 2.5.5 In addition, weigh six 50-g test portions of Materials 1, 4, and 6 for the OMA 990.28 reference method. 2.5.6 Randomize and blind code two OMA test portions of each material, bag and label the test portion sets for three collaborators. One of the collaborators can be the organizing laboratory. 2.5.7 Freeze all test portions at -80°C. Shipment of Test Portions 2.6.1 Test portions will be packaged in leak-proof insulated containers and shipped on dry ice by overnight carrier to arrive on Tuesday before initiation of analysis .

2.6

6

DRAFT