OMA Protocol Review: OMAMAN-56 Sulfites in Shrimp

Biofish-300 SUL: AOAC Performance Tested Method SM 031802

Abstract The Biofish-300 SUL method is a highly specific enzymatic biosensor for the rapid quantification of sulfite, measured as SO 2 content, in crustacean. Sulfite in raw shrimp head on, head off and boiled shrimp was analyzed and performance was examined using naturally contaminated and spiked samples, by comparisons with OMA 990.28 methods. Linearity, selectivity, matrix, consistency, and robustness were evaluated. All results were within acceptable ranges except robustness, which reflected deviation in sample volume and ultraturrax time compared to the standard assay procedures. Accuracy, assessed as a comparison of the Biofish results to the OMA results, ranged from 82 to 115% in all samples except for fortified raw shrimp head on where the low level yielded an accuracy of 138 %. The method bias was in general negative in both incurred and fortified high levels, and slightly positive in incurred low levels. Repeatability was very good as shown by the low RDSr values demonstrating acceptable repeatability precision with results less than 10 % in most of the evaluated values. Regression analyses showed a good correlation between the Biofish and OMA methods with R 2 > 0.99 in all cases.

Method Authors Sandra Salleres, Irune González, Naiara Linaza, Erlantz Ramos, Mónica Fernández, Roberto González, Sonia Maza, and Arrate Jaureguibeitia

Submitting Company Biolan Microbiosensores S.L. Parque Tecnológico de Bizkaia, Laida kalea, 409. 48170 Zamudio, Vizcaya. Spain. biolanmb@biolanmb.com (+34) 946 57 41 61

Independent Laboratory Eurofins Nutritional Analysis Center- Des Moines, IA 2220 Rittenhouse St., Des Moines, IA 50321, USA Phone: 515-265-1461 Contract Laboratory Anfaco Cecopesca Crta. Colexio Universitario, 16, 36310 Vigo - Pontevedra contacto@anfaco.es (+34)986469301

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