OMA Protocol Review: OMAMAN-56 Sulfites in Shrimp

Materials and Methods

Test Kit Information

a) Kit Name .—Biofish-300 SUL b) Catalog Number .— BF004 c) Ordering Information i. Worldwide. –

Biolan Microbiosensores S.L. Parque Tecnológico de Vizcaya, Laida kalea, 409. 48170 Zamudio, Vizcaya. Spain

(+34) 946 57 41 61 www.biolanmb.com biolanmb@biolanmb.com

d) Test Kit Components i. BTFSUL20: SUL Biotest for 20 analyses + SUL1 Extraction Reagent (SEFSUL1) for 20 analyses. ii. BTFSUL50: SUL Biotest for 50 analyses + SUL1 Extraction Reagent (SEFSUL1) for 50 analyses. iii. BTFSUL100: SUL Biotest for 100 analyses + SUL2 Extraction Reagent (SEFSUL2) for 100 analyses. iv. BTFSUL125: SUL Biotest for 125 analyses + SUL2 Extraction Reagent (SEFSUL2) for 125 analyses. v. KITCFSUL: SUL Calibration Kit (4 tubes with Calibration Reagent (CFSUL)). vi. KITMFSUL1: SUL1 Measurement Kit (8 tubes of Measurement Reagent (MFSUL1) for a total of 160 analyses). vii. KITMFSUL2: SUL2 Measurement Kit (5 tubes of Measurement Reagent (MFSUL2) for a total of 450 analyses).

viii. ELEREFN: Reference electrode and storage solution (SOLALMER). ix. KITVFSUL: SUL Verifying Kit (4 tubes of Verifying Reagent (VFSUL).

e) Additional supplies and reagents i. Distilled water

ii. Plastic tube .-Falcon, 50 mL iii. Plastic Test tube.- 25 mL

Apparatus

a) Biofish Biosensor .-BF300-004: Biofish 300 sulfite. b) Analytical balance. -Capable of measurement with two decimal places (g). c) Mincer.- d) Sample homogenizer .-Ultraturrax IKA T-25, or equivalent. e) Adjustable pipettes .-Capable of delivering 20-200 μL and 500-5000 μL.

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