RI-ERP-FINALACTION-Recommendations

OMAMAN-20 C/ In House Validation Report ERP Use Only - March 2015

4.1.6.3

Spiked samples (non-processed and processed)

All four materials were tested at five different spiking concentration levels (including zero) with 20 repeats and one extraction per repeat (also 20 separate extractions in total).

Table 12 shows the results for rice flour (ethanol extraction). This is the only case were the POD response curve included a POD50% level. Nevertheless, it was decided not to include a POD response curve due to the limited additional information. No false-positives were observed.

Table 12. Results for rice flour spiked with WGPAT Gliadin (ethanol extraction); 20 replicates and 20 separate extractions per concentration

mg gliadin/kg

result for each repeat

POD

- - - - - - - -

- - - - - - - -

- - - - - - - -

- - - - -

-

-

0,00

0,00

- -

-

-

- -

0,50

0,00

- -

- - - -

+ +

+ +

+ + +

+

- - -

1,00

0,45

- -

- -

-

+

+ + + + + + + + + + + +

+ + + + + + + + + + + +

+ + + + + + + + + + + +

+ + + + + + + + + + + + + + + + + + + + + + + +

2,00

1,00

4,00

1,00

8,00

1,00

AOAC Research Institute Expert Review Panel Use Only

Table 13 revealed a very clear result for spiked soy flour (ethanol extraction). There is a clear cut-off at 1 mg gliadin per kg flour and no false-positives were observed.

In summary, both matrices behave quite comparable after ethanol extraction and it was once again confirmed that the dip-stick shows a very narrow concentration range where positive and negative results occur for one concentration.

Page 22 of 27

RIDA®QUICK Gliadin Validation report 2015-01-14