Review Team (February 2016)

Amino-03 (February 2016) FOR ERP USE ONLY DO NOT DISTRIBUTE

Amino Acids in Infant Formula and Adult/Pediatric Formulas Ultra High Performance Liquid Chromatography

f) Calculations:

Area of std. * Conc. of ISTD in μM

Relative Response factor (RRF) =

ISTD Area * Conc. of std. in μM

- A b

) * W AA

* C ISTD

* V s

* DF S

(A s

Amino acid (g/100g) =

* 1000 * W s

* 10000

RRF * A ISTD

= Sample area = Blank area

A s A b

= Molecular weight of corresponding amino acid.

W AA A ISTD C ISTD

= ISTD area of sample. = Conc. of ISTD in μM = Sample Makeup Volume = Sample weight in grams

V s W s

= Sample dilution factor (Other than V s and W s )

DF S

1000 = Conversion factor for μM to mM 10000 =Conversion factor for mg/kg to g/100g

G. References: a) UPLC Amino acid analysis solution system guide. Waters

b) ISO 13904:2005(E) : Animal Feeding stuffs – Determination of Tryptophan content. c) ISO 13903:2005- : Animal Feeding Stuffs – Determination of Amino acids Content. d) Sulfur amino acids in food, feed ingredients and processed foods – AOAC 985.28 e) Amino acids in Feeds – AOAC 994.12

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