Review Team (February 2016)

BVit-03 (February 2016) FOR ERP USE ONLY DO NOT DISTRIBUTE

AOAC- SPIFAN-2012-VITAMINS

Validation Parameter

Limit of Quantification

Test Group Name

Date

2015\January\Vitamins\01.01.2015 01.01.2015

B-Complex vitamins Pantothenic acid

Test Name

Data Path File

Matrix

Raw-Data File Ref. CRM Data File Ref.

TUV/BLR/F-360 TUV/BLR/F-99

Aqueous

Limit

Equipment Test Method

Analyst

Prasad BKVR/Kesav Kumar N Manjunath K/Prasad BKVR

LC-MS/MS 6460A (BLR/PS-CRS/006.03)

Operator

Repeatability at LOQ 5 ug/l standard Sample Blank No.

Mean Retention Time (min)

m/z

Signal (ug/l)

Caluclation

220.1>90.0 220.1>90.0 220.1>90.0 220.1>90.0 220.1>90.0 220.1>90.0 220.1>90.0 220.1>90.0 220.1>90.0 220.1>90.0

2.843 2.843 2.843 2.843 2.843 2.843 2.843 2.843 2.843 2.843

4.437 3.960 4.141 4.110 4.335 4.361 4.482 4.147 4.086 4.229

1 2 3 4 5 6 7 8 9

Mean (μg/l)

4.23

SD

0.1689

LOD (Mean +3 sigma) LOQ (Mean + 10sigma)

4.74 5.92 3.99

RSD %

5

Standard Conc. (μg/l)

84.6

Accuracy %

Accuracy = 70 - 125 %

Criteria

10

RSD% = <15%

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