Review Team (February 2016)

BVit-03 (February 2016) FOR ERP USE ONLY DO NOT DISTRIBUTE

AOAC- SPIFAN-2012-VITAMINS

Validation Parameter

Limit of Quantification

Test Group Name

Date

2015\January\Vitamins\01.01.2015 01.01.2015

B-Complex vitamins Nicotinic acid

Test Name

Data Path File

Matrix

Raw-Data File Ref. CRM Data File Ref.

TUV/BLR/F-360 TUV/BLR/F-99

Aqueous

Limit

Equipment Test Method

Analyst

Prasad BKVR/Kesav Kumar N Manjunath K/Prasad BKVR

LC-MS/MS 6460A (BLR/PS-CRS/006.03)

Operator

Repeatability at LOQ 5 ug/l standard Sample Blank No.

Mean Retention Time (min)

m/z

Signal (ug/l)

Caluclation

124.2>80.1 124.2>80.1 124.2>80.1 124.2>80.1 124.2>80.1 124.2>80.1 124.2>80.1 124.2>80.1 124.2>80.1 124.2>80.1

1.867 1.867 1.867 1.867 1.867 1.867 1.867 1.867 1.867 1.867

5.617 5.533 4.943 5.486 6.255 5.994 5.832 5.271 5.301 5.611

1 2 3 4 5 6 7 8 9

Mean (μg/l)

5.58

SD

0.3774

LOD (Mean +3 sigma) LOQ (Mean + 10sigma)

6.72 9.36 6.76

RSD %

5

Standard Conc. (μg/l)

111.7

Accuracy %

Accuracy = 70 - 125 %

Criteria

10

RSD% = <15%

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