Review Team (February 2016)

BVit-03 (February 2016) FOR ERP USE ONLY DO NOT DISTRIBUTE

AOAC- SPIFAN-2012-VITAMINS

Validation Parameter

Limit of Quantification

Test Group Name

Date

01.01.2015 2015\January\Vitamins\01.01.2015 Prasad BKVR/Kesav Kumar N TUV/BLR/F-360 TUV/BLR/F-99 Manjunath K/Prasad BKVR

B-Complex vitamins Niacinamide Aqueous

Test Name

Data Path File

Matrix

Raw-Data File Ref. CRM Data File Ref.

Limit

Equipment Test Method

Analyst

LC-MS/MS 6460A (BLR/PS-CRS/006.03)

Operator

Repeatability at LOQ 5 ug/l standard Sample Blank No.

Mean Retention Time (min)

m/z

Signal (ug/l)

Caluclation

123.2>80.3 123.2>80.3 123.2>80.3 123.2>80.3 123.2>80.3 123.2>80.3 123.2>80.3 123.2>80.3 123.2>80.3 123.2>80.3

2.315 2.315 2.315 2.315 2.315 2.315 2.315 2.315 2.315 2.315

4.949 4.916 4.539 4.639 4.896 4.580 4.966 4.491 4.725 4.555

1 2 3 4 5 6 7 8 9

Mean (μg/l)

4.73

SD

0.1886

LOD (Mean +3 sigma) LOQ (Mean + 10sigma)

5.29 6.61 3.99

RSD %

5

Standard Conc. (μg/l)

94.5

Accuracy %

Accuracy = 70 - 125 %

Criteria

10

RSD% = <15%

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